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Genomic characterization of a novel human papillomavirus (HPV-117) with a high viral load in a persisting wart
- Source :
- Virology. (1):129-133
- Publisher :
- Elsevier Inc.
-
Abstract
- Warts from immunosuppressed organ transplant recipients (OTR) persist over years and may progress into non-melanoma skin cancer. Human papillomaviruses (HPV) are considered the causal agents for the development of such warts. We isolated the novel type HPV-117 from a persisting wart by rolling circle amplification. One hundred eighteen warts from immunocompetent patients (IC) and 49 warts from OTR were analyzed by HPV-117 E6 type-specific PCR. As inferred from a phylogenetic analysis, the new type HPV-117 belonged to alpha-PV species 2, including the most similar types HPV-10 and HPV-94. The general prevalence of HPV-117 in warts was 2% in IC (2/118), and 12% in OTR (6/49). The high viral load in dysplastic cells of a Verruca vulgaris was shown by in situ hybridization. Our results suggest an active role of the novel type in the development of cutaneous warts of OTR.
- Subjects :
- Adult
Male
medicine.medical_specialty
Molecular Sequence Data
Prevalence
In situ hybridization
Genome, Viral
Cutaneous warts
Biology
Organ transplantation
Immunocompromised Host
Viral Envelope Proteins
Virology
medicine
Humans
Human papillomavirus
Papillomaviridae
In Situ Hybridization
Phylogeny
Human papillomavirus (HPV)
Reverse Transcriptase Polymerase Chain Reaction
Papillomavirus Infections
virus diseases
Organ transplant recipients (OTR)
Viral Load
medicine.disease
Rolling circle replication
Immunology
Skin cancer
Warts
Viral load
Sequence Alignment
Verruca Vulgaris
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....af0f9ff5dfc9d42577100698f0880eb3
- Full Text :
- https://doi.org/10.1016/j.virol.2009.12.023