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Multiscale investigation of USPIO nanoparticles in atherosclerotic plaques and their catabolism and storage in vivo
- Source :
- Nanomedicine: Nanotechnology, Biology and Medicine, Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2016, 12, pp.191-200. ⟨10.1016/j.nano.2015.08.005⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- The storage and catabolism of Ultrasmall SuperParamagnetic Iron Oxide (USPIO) nanoparticles were analyzed through a multiscale approach combining Two Photon Laser Scanning Microscopy (TPLSM) and High-Resolution Transmission Electron Microscopy (HRTEM) at different times after intravenous injection in an atherosclerotic ApoE(-/-) mouse model. The atherosclerotic plaque features and the USPIO heterogeneous biodistribution were revealed down from organ's scale to subcellular level. The biotransformation of the nanoparticle iron oxide (maghemite) core into ferritin, the non-toxic form of iron storage, was demonstrated for the first time ex vivo in atherosclerotic plaques as well as in spleen, the iron storage organ. These results rely on an innovative spatial and structural investigation of USPIO's catabolism in cellular phagolysosomes. This study showed that these nanoparticles were stored as non-toxic iron compounds: maghemite oxide or ferritin, which is promising for MRI detection of atherosclerotic plaques in clinics using these USPIOs. From the Clinical Editor: Advance in nanotechnology has brought new contrast agents for clinical imaging. In this article, the authors investigated the use and biotransformation of Ultrasmall Super-paramagnetic Iron Oxide (USPIO) nanoparticles for analysis of atherosclerotic plagues in Two Photon Laser Scanning Microscopy (TPLSM) and High-Resolution Transmission Electron Microscopy (HRTEM). The biophysical data generated from this study could enable the possible use of these nanoparticles for the benefits of clinical patients.
- Subjects :
- 0301 basic medicine
Biodistribution
Pathology
medicine.medical_specialty
Materials science
Metabolic Clearance Rate
MRI contrast agent
Biomedical Engineering
Iron oxide
Contrast Media
Pharmaceutical Science
Medicine (miscellaneous)
Bioengineering
02 engineering and technology
Mice
03 medical and health sciences
chemistry.chemical_compound
[SPI]Engineering Sciences [physics]
Microscopy, Electron, Transmission
Two-photon excitation microscopy
In vivo
Materials Testing
medicine
Animals
[CHIM]Chemical Sciences
Tissue Distribution
General Materials Science
Magnetite Nanoparticles
High-resolution transmission electron microscopy
ComputingMilieux_MISCELLANEOUS
[PHYS]Physics [physics]
biology
Dextrans
021001 nanoscience & nanotechnology
Plaque, Atherosclerotic
Mice, Inbred C57BL
Ferritin
Metabolism
Microscopy, Fluorescence, Multiphoton
030104 developmental biology
chemistry
biology.protein
Molecular Medicine
0210 nano-technology
Ex vivo
Subcellular Fractions
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 15499634
- Database :
- OpenAIRE
- Journal :
- Nanomedicine: Nanotechnology, Biology and Medicine, Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2016, 12, pp.191-200. ⟨10.1016/j.nano.2015.08.005⟩
- Accession number :
- edsair.doi.dedup.....af06cc78852b16d4962be76da9edaaad