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Predominant clonal accumulation of CD8+ T cells with moderate avidity in the central nervous systems of Theiler's virus-infected C57BL/6 mice
- Source :
- Journal of virology. 84(6)
- Publication Year :
- 2010
-
Abstract
- Induction of antigen-specific CD8+T cells bearing a high-avidity T-cell receptor (TCR) is thought to be an important factor in antiviral and antitumor immune responses. However, the relationship between TCR diversity and functional avidity of epitope-specific CD8+T cells accumulating in the central nervous system (CNS) during viral infection is unknown. Hence, analysis of T-cell diversity at the clonal level is important to understand the fate and function of virus-specific CD8+T cells. In this study, we examined the Vβ diversity and avidity of CD8+T cells specific to the predominant epitope (VP2121-130) of Theiler's murine encephalomyelitis virus. We found that Vβ6+CD8+T cells, associated with epitope specificity, predominantly expanded in the CNS during viral infection. Further investigations of antigen-specific Vβ6+CD8+T cells by CDR3 spectratyping and sequencing indicated that distinct T-cell clonotypes are preferentially increased in the CNS compared to the periphery. Among the epitope-specific Vβ6+CD8+T cells, MGX-Jβ1.1 motif-bearing cells, which could be found at a high precursor frequency in naïve mice, were expanded in the CNS and tightly associated with gamma interferon production. These T cells displayed moderate avidity for the cognate epitope rather than the high avidity normally observed in memory/effector T cells. Therefore, our findings provide new insights into the CD8+T-cell repertoire during immune responses to viral infection in the CNS.
- Subjects :
- Central Nervous System
Receptors, Antigen, T-Cell, alpha-beta
Immunology
Molecular Sequence Data
chemical and pharmacologic phenomena
Biology
CD8-Positive T-Lymphocytes
Microbiology
Epitope
Interleukin 21
Epitopes
Mice
Immune system
Theilovirus
Virology
Cardiovirus Infections
Cytotoxic T cell
Animals
Humans
Avidity
IL-2 receptor
Amino Acid Sequence
T-cell receptor
Mice, Inbred C57BL
Insect Science
Pathogenesis and Immunity
CD8
Spleen
Subjects
Details
- ISSN :
- 10985514
- Volume :
- 84
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of virology
- Accession number :
- edsair.doi.dedup.....aed4de880b1df86d4b3b9b613e3b3f52