Back to Search
Start Over
Tautomeric G•U pairs within the molecular ribosomal grip and fidelity of decoding in bacteria
- Source :
- Nucleic Acids Research, Nucleic Acids Research, 2018, 46 (14), pp.7425-7435. ⟨10.1093/nar/gky547⟩, Nucleic Acids Research, Oxford University Press, 2018, 46 (14), pp.7425-7435. ⟨10.1093/nar/gky547⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- International audience; We report new crystallographic structures of Thermus thermophilus ribosomes complexed with long mRNAs and native Escherichia coli tRNAs. They complete the full set of combinations of Watson-Crick G•C and miscoding G•U pairs at the first two positions of the codon-anticodon duplex in ribosome functional complexes. Within the tight decoding center, miscoding G•U pairs occur, in all combinations, with a non-wobble geometry structurally indistinguishable from classical coding Watson-Crick pairs at the same first two positions. The contacts with the ribosomal grip surrounding the decoding center are all quasi-identical, except in the crowded environment of the amino group of a guanosine at the second position; in which case a G in the codons may be preferred. In vivo experimental data show that the translational errors due to miscoding by G•U pairs at the first two positions are the most frequently encountered ones, especially at the second position and with a G on the codon. Such preferred miscodings involve a switch from an A-U to a G•U pair in the tRNA/mRNA complex and very rarely from a G = C to a G•U pair. It is concluded that the frequencies of such occurrences are only weakly affected by the codon/anticodon structures but depend mainly on the stability and lifetime of the complex, the modifications present in the anticodon loop, especially those at positions 34 and 37, in addition to the relative concentration of cognate/near-cognate tRNA species present in the cellular tRNA pool.
- Subjects :
- Models, Molecular
0301 basic medicine
Base pair
Stereochemistry
[SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
[SDV]Life Sciences [q-bio]
Guanosine
Biology
Crystallography, X-Ray
Ribosome
03 medical and health sciences
chemistry.chemical_compound
RNA, Transfer
Structural Biology
Anticodon
Escherichia coli
Genetics
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
RNA, Messenger
Codon
Base Pairing
ComputingMilieux_MISCELLANEOUS
030102 biochemistry & molecular biology
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
Thermus thermophilus
RNA
Ribosomal RNA
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
030104 developmental biology
chemistry
Duplex (building)
Protein Biosynthesis
Transfer RNA
Nucleic Acid Conformation
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Ribosomes
Subjects
Details
- Language :
- English
- ISSN :
- 03051048 and 13624962
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research, Nucleic Acids Research, 2018, 46 (14), pp.7425-7435. ⟨10.1093/nar/gky547⟩, Nucleic Acids Research, Oxford University Press, 2018, 46 (14), pp.7425-7435. ⟨10.1093/nar/gky547⟩
- Accession number :
- edsair.doi.dedup.....aed19426d01d902bd1b1407a3c0bdeb1