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Isolation of a single-stranded plasmid from Clostridium acetobutylicum NCIB 6444
- Source :
- Europe PubMed Central
- Publication Year :
- 1990
- Publisher :
- American Society for Microbiology, 1990.
-
Abstract
- The cryptic plasmid pDM6 was isolated from late exponential-phase cells of Clostridium acetobutylicum NCIB 6444 by either alkaline lysis or electroporation. The application of high voltage during electroporation resulted in higher DNA yield than did the alkaline lysis procedure. However, electroporation-induced plasmid release generated high amounts of single-stranded DNA compared with the alkaline lysis procedure, which generated both double-stranded DNA (monomer and dimer forms) and single-stranded DNA.
- Subjects :
- Clostridium
DNA, Bacterial
Plasmid preparation
Clostridium acetobutylicum
Lysis
Ecology
biology
Electroporation
DNA, Single-Stranded
Hydrogen-Ion Concentration
biology.organism_classification
Applied Microbiology and Biotechnology
Molecular biology
chemistry.chemical_compound
Plasmid
Biochemistry
chemistry
Electrochemistry
Alkaline lysis
DNA
Plasmids
Research Article
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 10985336 and 00992240
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....aecc764e75b0db216d1bf5615dbf01be