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Detection of antibodies to West Nile virus in equine sera using microsphere immunoassay
- Source :
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 18(4)
- Publication Year :
- 2006
-
Abstract
- One hundred and ninety-one sera from horses that recently were exposed to West Nile virus (WNV) by either vaccination or natural infection or that were not vaccinated and remained free of infection were used to evaluate fluorescent microsphere immunoassays (MIAs) incorporating recombinant WNV envelope protein (rE) and recombinant nonstructural proteins (rNS1, rNS3, and rNS5) for detection of equine antibodies to WNV. The rE MIA had a diagnostic sensitivity and specificity, respectively, of 99.3% and 97.4% for detection of WNV antibodies in the serum of horses that were recently vaccinated or naturally infected with WNV, as compared to the plaque reduction neutralization test (PRNT). The positive rE MIA results were assumed to be WNV-specific because of the close agreement between this assay and the PRNT and the fact that unvaccinated control horses included in this study were confirmed to be free of exposure to the related St Louis encephalitis virus. The NS protein–based MIA were all less sensitive than either the rE MIA or PRNT (sensitivity 0–48.0), although the rNS1 MIA distinguished horses vaccinated with the recombinant WNV vaccine from those that were immunized with the inactivated WNV vaccine ( P < 0.0001) or naturally infected with WNV ( P < 0.0001). The rE MIA would appear to provide a rapid, convenient, inexpensive, and accurate test for the screening of equine sera for the presence of antibodies to WNV.
- Subjects :
- 0301 basic medicine
endocrine system diseases
040301 veterinary sciences
West Nile virus
animal diseases
viruses
030106 microbiology
medicine.disease_cause
Antibodies, Viral
Serology
law.invention
0403 veterinary science
03 medical and health sciences
Plaque reduction neutralization test
law
medicine
Animals
Horses
Immunoassay
General Veterinary
medicine.diagnostic_test
biology
business.industry
virus diseases
Horse
Viral Vaccines
04 agricultural and veterinary sciences
Virology
Microspheres
nervous system diseases
Vaccination
Recombinant DNA
biology.protein
Horse Diseases
Antibody
business
West Nile Fever
Subjects
Details
- ISSN :
- 10406387
- Volume :
- 18
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Accession number :
- edsair.doi.dedup.....aeb60dadb191c12fb3553097242540d4