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The fragment of complement 3a lacking the primary 9 amino acids induces promoting activity on mouse voluntary running
- Source :
- The Tohoku journal of experimental medicine. 189(1)
- Publication Year :
- 2000
-
Abstract
- We have newly found that interleukin-2 (IL-2) increases mouse voluntary running 24 hours, but not 30 minutes, after the injection. We suspected that IL-2 induced a substance increasing the voluntary running for 24 hours after injection. Serum obtained from mice 24 hours after the IL-2 treatment was fractioned with the use of an ion-exchanger and an ultra-filtration method, and the amino acid sequence analysis indicated that the substance purified from the effective fraction was a fragment of mouse complement 3a (C3a) lacking the primary 9 amino acids. The 20 amino acid peptide synthesized according to the fragment showed the activity increasing the voluntary running, but the 20 amino acid peptide synthesized according to the C3a itself did not. The effect of the synthesized peptide was demuted by haloperidol but not by a specific dopamine 2 antagonist (−)sulpiride. The present findings clearly indicate that IL-2 produces the C3a fragment lacking the primary 9 amino acids which directly promotes the voluntary running, and that the effect of the fragment is mediated by an activity of haloperidol on the neurons, except for the dopamine 2 antagonism.
- Subjects :
- Male
Molecular Sequence Data
Physical Exertion
Peptide
Biology
General Biochemistry, Genetics and Molecular Biology
Running
Mice
Structure-Activity Relationship
Dopamine
medicine
Haloperidol
Animals
Amino Acid Sequence
Peptide sequence
Sequence Deletion
chemistry.chemical_classification
Antagonist
General Medicine
Peptide Fragments
Amino acid
chemistry
Biochemistry
Complement C3a
Interleukin-2
Antagonism
Sulpiride
medicine.drug
Subjects
Details
- ISSN :
- 00408727
- Volume :
- 189
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Tohoku journal of experimental medicine
- Accession number :
- edsair.doi.dedup.....ae8e373335a875390bf3b1c43cea5f15