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Molecular characterization of rotavirus strains from children in Toronto, Canada
- Source :
- Journal of Clinical Virology. 28:77-84
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- Background: Rotaviruses are the most common agents of diarrheal illness of infants and young children. Gastroenteritis caused by rotaviruses is also more likely to be associated with severe dehydration compared to other viral gastroenteritis. Objectives: We determined the G-serotype of rotaviruses circulating in the Metro Toronto-Peel County region, during the 1997–1998 winter. Study design: Stool samples were collected in Metro Toronto-Peel county region from November 1, 1997 to June 30, 1998. Rotavirus presence was demonstrated by electron microscopy and ELISA. A reverse transcription-polymerase chain reaction (RT-PCR) assay for the amplification of the near-complete RHA segment 9 of rotavirus was developed and used to determine the G-serotype by sequencing and phylogenetic analysis. Results: A total of 135 rotavirus isolates were used as templates for RT-PCR. Of these, four could not be amplified and one revealed a mixed infection. The G-serotypes could be unambiguously determined for the other isolates. Of these, 85 were of G-serotype 1, 41 of G2, 1 of G3, 1 of G4, and 2 of G9. Conclusion: Our RT-PCR assay enables unambiguous determination of the G-serotype. The overall relative incidence of G-serotypes in Toronto is similar to that of other developed countries. Serotype G9 was present at low levels in Toronto.
- Subjects :
- Rotavirus
Serotype
Canada
Relative incidence
Rotavirus Infections
Biology
medicine.disease_cause
Severe dehydration
Microbiology
Virology
medicine
Humans
Serotyping
Child
Antigens, Viral
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Infectious Diseases
Child, Preschool
RNA, Viral
Capsid Proteins
Mixed infection
Subjects
Details
- ISSN :
- 13866532
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Virology
- Accession number :
- edsair.doi.dedup.....ae881e2e26354924c4ab5e60c281cff8
- Full Text :
- https://doi.org/10.1016/s1386-6532(02)00266-4