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IL-10 Has A Distinct Immunoregulatory Effect on Naive and Active T Cell Subsets
- Source :
- Journal of Interferon & Cytokine Research. 27:1031-1038
- Publication Year :
- 2007
- Publisher :
- Mary Ann Liebert Inc, 2007.
-
Abstract
- Interleukin-10 (IL-10) has been identified as a key immunomodulatory cytokine on T cells. However, both immunosuppressive and immunostimulatory effects of IL-10 on T cells also have been reported. The discrepancy between these in vitro effects of IL-10 may be due to the different T cells (naive vs. active or resting active T cells) used under various experimental conditions in these studies. Therefore, it is necessary to clearly define the IL-10 effect on T cell subsets in their different statuses. In this study, we used a molecularly defined T cell system, the ovalbumin (OVA)-specific CD4(+) and CD8(+) T cells from transgenic OT-I and OT-II mice expressing OVA-specific T cell receptor (TCR). We investigated the effect of IL-10 on these OVA-specific T cell subsets in their different statuses (i.e., naive and active T cells). Our data demonstrate that IL-10 has distinct immunoregulatory effects on naive and active T cell subsets. IL-10 inhibits active CD4(+) T cell proliferation, whereas it stimulates and suppresses active CD8(+) T cell proliferation and cytotoxicity, respectively. IL-10-treated dendritic cells (DCs) stimulate anergic cytotoxic T lymphocyte-associated molecule-4 (CTLA)-4-expressing CD4(+) T cell responses possibly through downregulation of major histocompetibility complex (MHC) class II and costimulatory molecule expression on DCs. The anergic CD4(+) T cells suppress T cell proliferation mainly through a CTLA-4-mediated pathway. The distinct role of IL-10 on T cell subsets may be useful in designing T cell-based immunotherapy of cancer and infectious diseases.
- Subjects :
- CD4-Positive T-Lymphocytes
Cytotoxicity, Immunologic
Ovalbumin
T cell
Immunology
Mice, Transgenic
CD8-Positive T-Lymphocytes
Biology
Lymphocyte Activation
Mice
Interleukin 21
Antigens, CD
T-Lymphocyte Subsets
Virology
medicine
Animals
Cytotoxic T cell
CTLA-4 Antigen
Receptors, Interleukin-10
IL-2 receptor
Antigen-presenting cell
Cells, Cultured
ZAP70
Histocompatibility Antigens Class II
CD28
Dendritic Cells
Cell Biology
Natural killer T cell
Antigens, Differentiation
Interleukin-10
Cell biology
medicine.anatomical_structure
Cytokines
Immunization
Subjects
Details
- ISSN :
- 15577465 and 10799907
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Journal of Interferon & Cytokine Research
- Accession number :
- edsair.doi.dedup.....ae7ec4ebc084bf7dbd87a73e7f57c054