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A xylan glucuronosyltransferase gene exhibits pleiotropic effects on cellular composition and leaf development in rice
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Leaf chlorophyll content is an important physiological indicator of plant growth, metabolism and nutritional status, and it is highly correlated with leaf nitrogen content and photosynthesis. In this study, we report the cloning and identification of a xylan glucuronosyltransferase gene (OsGUX1) that affects relative chlorophyll content in rice leaf. Using a set of chromosomal segment substitution lines derived from a cross of wild rice accession ACC10 and indica variety Zhenshan 97 (ZS97), we identified numerous quantitative trait loci for relative chlorophyll content. One major locus of them for relative chlorophyll content was mapped to a 10.3-kb region that contains OsGUX1. The allele OsGUX1AC from ACC10 significantly decreases nitrogen content and chlorophyll content of leaf compared with OsGUX1ZS from ZS97. The overexpression of OsGUX1 reduced chlorophyll content, and the suppression of this gene increased chlorophyll content of rice leaf. OsGUX1 is located in Golgi apparatus, and highly expressed in seedling leaf and the tissues in which primary cell wall synthesis occurring. Our experimental data indicate that OsGUX1 is responsible for addition of glucuronic acid residues onto xylan and participates in accumulation of cellulose and hemicellulose in the cell wall deposition, thus thickening the primary cell wall of mesophyll cells, which might lead to reduced chlorophyll content in rice leaf. These findings provide insights into the association of cell wall components with leaf nitrogen content in rice.
- Subjects :
- Chlorophyll
0106 biological sciences
0301 basic medicine
Glucuronosyltransferase
Nitrogen
Quantitative Trait Loci
lcsh:Medicine
Locus (genetics)
Photosynthesis
01 natural sciences
Article
Plant breeding
Cell wall
03 medical and health sciences
chemistry.chemical_compound
Glucuronic Acid
Cell Wall
Botany
Hemicellulose
Cellulose
lcsh:Science
Nitrogen cycle
Alleles
Plant Proteins
Multidisciplinary
biology
fungi
lcsh:R
food and beverages
Oryza
biology.organism_classification
Plant Leaves
030104 developmental biology
chemistry
Seedlings
Seedling
biology.protein
Hybridization, Genetic
Xylans
lcsh:Q
Gene expression
010606 plant biology & botany
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....ae68c7bf35eaa8f38d6fa5e7818532a5