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Three dimensional cultivation increases chemo- and radioresistance of colorectal cancer cell lines
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 1, p e0244513 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science, 2021.
-
Abstract
- Although 2D cell cultures are commonly used to predict therapy response, it has become clear that 3D cultures may better mimic the in vivo situation and offer the possibility of tailoring translational clinical approaches. Here, we compared the response of 2D and 3D colorectal cancer (CRC) cell lines to irradiation and chemotherapy. Classic 2D cultures and 3D spheroids of CRC cell lines (CaCo2, Colo205, HCT116, SW480) were thoroughly established, then irradiated with doses of 1, 4, or 10 Gy, using a clinical-grade linear accelerator. The response was assessed by immunohistochemistry, flow cytometry, and TUNEL assays. Upon irradiation, CRC 3D spheroids were morphologically altered. After irradiation with 10 Gy, annexin V/PI staining revealed a 1.8- to 4-fold increase in the apoptosis rate in the 2D cell cultures (95% CI 3.24±0.96), and a 1.5- to 2.4-fold increase in the 3D spheroids (95% CI 1.56±0.41). Irradiation with 1 Gy caused 3- and 4-fold increases in TUNEL positive cells in the CaCo2 and HCT116 (p = 0.01) 2D cultures, respectively, compared with a 2-fold increase in the 3D spheroids. Furthermore, the 2D and 3D cultures responded differently to chemotherapy; the 3D cultures were more resistant to 5-FU and cisplatin, but not to doxorubicin and mitomycin C, than the 2D cultures. Taken together, CRC cells cultured as 3D spheroids displayed markedly higher resistance to irradiation therapy and selected chemotherapeutic drugs than 2D cultures. This in vitro difference must be considered in future approaches for determining the ideal in vitro systems that mimic human disease.
- Subjects :
- medicine.medical_treatment
Cell Lines
Cancer Treatment
Cell Culture Techniques
Apoptosis
Radiation Tolerance
Radiation, Ionizing
Medicine and Health Sciences
Staining
Multidisciplinary
medicine.diagnostic_test
Cell Death
Chemistry
Pharmaceutics
Cell Staining
Oncology
Cell Processes
Medicine
Biological Cultures
Fluorouracil
Colorectal Neoplasms
medicine.drug
Research Article
Science
Antineoplastic Agents
Research and Analysis Methods
Flow cytometry
Drug Therapy
In vivo
Radioresistance
Cell Line, Tumor
Spheroids, Cellular
medicine
Chemotherapy
Humans
Doxorubicin
Cell Proliferation
Cisplatin
Colorectal Cancer
Cancers and Neoplasms
Biology and Life Sciences
Cell Biology
Cell Cultures
SW480 cells
Cell culture
Specimen Preparation and Treatment
Drug Resistance, Neoplasm
Cancer research
Caco-2 Cells
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....ae670b5c1da8dc31480310f2636204fc