Back to Search
Start Over
Platelets Proteomic Profiles of Acute Ischemic Stroke Patients
- Source :
- PLoS ONE, Vol 11, Iss 6, p e0158287 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- WOS: 000378389200128<br />PubMed ID: 27336623<br />Platelets play a crucial role in the pathogenesis of stroke and antiplatelet agents exist for its treatment and prevention. Through the use of LC-MS based protein expression profiling, platelets from stroke patients were analyzed and then correlated with the proteomic analyses results in the context of this disease. This study was based on patients who post ischemic stroke were admitted to hospital and had venous blood drawn within 24 hrs of the incidence. Label-free protein expression analyses of the platelets' tryptic digest was performed in triplicate on a UPLC-ESI-qTOF-MS/MS system and ProteinLynx Global Server (v2.5, Waters) was used for tandem mass data extraction. The peptide sequences were searched against the reviewed homo sapiens database (www.uniprot.org) and the quantitation of protein variation was achieved through Progenesis LC-MS software (V4.0, Nonlinear Dynamics). These Label-free differential proteomics analysis of platelets ensured that 500 proteins were identified and 83 of these proteins were found to be statistically significant. The differentially expressed proteins are involved in various processes such as inflammatory response, cellular movement, immune cell trafficking, cell-to-cell signaling and interaction, hematological system development and function and nucleic acid metabolism. The expressions of myeloperoxidase, arachidonate 12-Lipoxygenase and histidine-rich glycoprotein are involved in cellular metabolic processes, crk-like protein and ras homolog gene family member A involved in cell signaling with vitronectin, thrombospondin 1, Integrin alpha 2b, and integrin beta 3 involved in cell adhesion. Apolipoprotein H, immunoglobulin heavy constant gamma 1 and immunoglobulin heavy constant gamma 3 are involved in structural, apolipoprotein A-I, and alpha-1-microglobulin/bikunin precursor is involved in transport, complement component 3 and clusterin is involved in immunity proteins as has been discussed. Our data provides an insight into the proteins that are involved in the platelets' activation response during ischemic stroke. It could be argued that this study lays the foundation for future mechanistic studies.<br />Marmara University Research Unit Grants [SAG-C-DRP-010710-0216]<br />This research was supported by Marmara University Research Unit Grants SAG-C-DRP-010710-0216. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Subjects :
- 0301 basic medicine
Male
Proteomics
Platelet Aggregation
Proteome
Physiology
Protein Expression
lcsh:Medicine
Antiplatelet Therapy
Vascular Medicine
Biochemistry
COMPLEMENT
ACTIVATION
Animal Cells
Tandem Mass Spectrometry
Immune Physiology
Medicine and Health Sciences
Platelet
lcsh:Science
chemistry.chemical_classification
Multidisciplinary
Immune System Proteins
biology
Pharmaceutics
Hematology
Middle Aged
LEUKEMIA PATIENTS
Cell biology
Body Fluids
Stroke
Blood
Neurology
THROMBOSPONDIN-1
Vitronectin
Female
Anatomy
Cellular Types
Apolipoprotein H
Research Article
Platelets
Adult
Blood Platelets
VITRONECTIN
Cerebrovascular Diseases
Integrin
Immunology
Research and Analysis Methods
Antibodies
RICH GLYCOPROTEIN BINDING
MECHANISMS
03 medical and health sciences
Drug Therapy
Gene Expression and Vector Techniques
Humans
ALPHA(1)-MICROGLOBULIN/BIKUNIN PRECURSOR AMBP
Platelet activation
Molecular Biology Techniques
Blood Coagulation
Molecular Biology
Ischemic Stroke
Aged
Molecular Biology Assays and Analysis Techniques
Blood Cells
Clusterin
lcsh:R
Biology and Life Sciences
Proteins
Cell Biology
QUANTIFICATION
Platelet Activation
THROMBOSIS
030104 developmental biology
chemistry
biology.protein
lcsh:Q
Glycoprotein
Chromatography, Liquid
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....ae64fff3b3ca96f0708d5cdfdd002e0e