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The chloroplast metalloproteases VAR2 and EGY1 act synergistically to regulate chloroplast development in Arabidopsis
- Source :
- J Biol Chem
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Chloroplast development and photosynthesis require the proper assembly and turnover of photosynthetic protein complexes. Chloroplasts harbor a repertoire of proteases to facilitate proteostasis and development. We have previously used an Arabidopsis leaf variegation mutant, yellow variegated2 (var2), defective in thylakoid FtsH protease complexes, as a tool to dissect the genetic regulation of chloroplast development. Here, we report a new genetic enhancer mutant of var2, enhancer of variegation3–1 (evr3–1). We confirm that EVR3 encodes a chloroplast metalloprotease, reported previously as ethylene-dependent gravitropism-deficient and yellow-green1 (EGY1)/ammonium overly sensitive1 (AMOS1). We observed that mutations in EVR3/EGY1/AMOS1 cause more severe leaf variegation in var2–5 and synthetic lethality in var2–4. Using a modified blue-native PAGE system, we reveal abnormal accumulations of photosystem I, photosystem II, and light-harvesting antenna complexes in EVR3/EGY1/AMOS1 mutants. Moreover, we discover distinct roles of VAR2 and EVR3/EGY1/AMOS1 in the turnover of photosystem II reaction center under high light stress. In summary, our findings indicate that two chloroplast metalloproteases, VAR2/AtFtsH2 and EVR3/EGY1/AMOS1, function coordinately to regulate chloroplast development and reveal new roles of EVR3/EGY1/AMOS1 in regulating chloroplast proteostasis in Arabidopsis.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chloroplasts
Mutant
Arabidopsis
Plant Biology
Photosystem I
01 natural sciences
Biochemistry
03 medical and health sciences
ATP-Dependent Proteases
Etiolation
Arabidopsis thaliana
Amino Acid Sequence
Photosynthesis
Molecular Biology
Variegation
Base Sequence
Photosystem I Protein Complex
biology
Arabidopsis Proteins
Protein Stability
food and beverages
Membrane Proteins
Photosystem II Protein Complex
Cell Biology
biology.organism_classification
Cell biology
Chloroplast
030104 developmental biology
Proteostasis
Genetic Loci
Thylakoid
Mutation
Metalloproteases
Protein Multimerization
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1083351X and 00219258
- Volume :
- 295
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....ae51233dd87b1f43d3fa2ceed99efc57