Back to Search
Start Over
Microbial community responses to bioremediation treatments for the mitigation of low-dose anthracene in marine coastal sediments of Bizerte lagoon (Tunisia)
- Source :
- Environmental Science and Pollution Research, Environmental Science and Pollution Research, Springer Verlag, 2013, 20 (1), pp.300--310. ⟨10.1007/s11356-012-0860-x⟩, Environmental Science and Pollution Research, 2013, 20 (1), pp.300--310. ⟨10.1007/s11356-012-0860-x⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- International audience; Purpose: The main goals of this study were to investigate (1) the behavior of microbial communities in response to low-dose bioavailable anthracene addition in lightly contaminated sediment from Bizerte Lagoon and (2) the effects of bioremediation treatments on microbial biomass, activity, and community structure. Methods: Sediment microcosms amended with 1 ppm anthracene were incubated in triplicate during 30 days. Biostimulation (addition of nitrogen and phosphorus fertilizer) and bioaugmentation (inoculation of a hydrocarbonoclastic bacterium) were used as bioremediation treatments. Bacterial biomass was estimated using flow cytometry. Sediment oxygen consumption was measured with oxygen microelectrodes. Bacterial community structure was assessed by molecular fingerprints (terminal restriction fragment length polymorphism; T-RFLP) analysis. Results: Anthracene contamination resulted in a significant reduction of bacterial abundance with an impact on cell integrity. Concomitantly, sediment oxygen consumption was strongly inhibited. Correspondence analysis on T-RFLP data indicated that bacterial community structures from anthracene-contaminated microcosms were different from that of the control. Interestingly, the changes observed in microbial biomass, structure, and activities as a result of anthracene contamination were not alleviated even with the use of biostimulation and combination of biostimulation and bioaugmentation strategy for anthracene bioremediation. Nevertheless, both treatment methods resulted in different community structures relative to the contaminated and control microcosms with the appearance of distinct populations. Conclusion: Anthracene spiking severely affected microbial communities, suggesting dominance of nontolerant populations in this lightly-contaminated sediment. Although biostimulation and/or bioaugmentation treatments did not alleviate the anthracene toxic effects, the changes observed in microbial population and structure suggest that the proposed treatments might be promising to promote bacterial growth. Further works are still required to propose a more efficient strategy to stimulate biodegradation that takes into account the complex interactions between species for resource access. © 2012 Springer-Verlag.
- Subjects :
- Geologic Sediments
Bioaugmentation
Tunisia
Microcosms
Health, Toxicology and Mutagenesis
Population
Microbial communities
010501 environmental sciences
Bacterial growth
01 natural sciences
Biostimulation
03 medical and health sciences
Bioremediation
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Environmental Chemistry
[CHIM]Chemical Sciences
Biomass
14. Life underwater
Fertilizers
education
030304 developmental biology
0105 earth and related environmental sciences
Anthracenes
0303 health sciences
education.field_of_study
Bacteria
Ecology
Chemistry
Oxygen microprofiles
Bizerte
General Medicine
[CHIM.MATE]Chemical Sciences/Material chemistry
Pollution
6. Clean water
lagoon
T-RFLP analysis
Terminal restriction fragment length polymorphism
Biodegradation, Environmental
Microbial population biology
13. Climate action
Environmental chemistry
Anthracene
Bizerte Lagoon
Water Microbiology
Microcosm
Polymorphism, Restriction Fragment Length
Water Pollutants, Chemical
Subjects
Details
- Language :
- English
- ISSN :
- 09441344 and 16147499
- Database :
- OpenAIRE
- Journal :
- Environmental Science and Pollution Research, Environmental Science and Pollution Research, Springer Verlag, 2013, 20 (1), pp.300--310. ⟨10.1007/s11356-012-0860-x⟩, Environmental Science and Pollution Research, 2013, 20 (1), pp.300--310. ⟨10.1007/s11356-012-0860-x⟩
- Accession number :
- edsair.doi.dedup.....ae1a79c61aa17e54bb7df2372fac3a69