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Mannoproteins from Saccharomyces cerevisiae stimulate angiogenesis by promoting the akt-eNOS signaling pathway in endothelial cells
- Source :
- Biochemical and Biophysical Research Communications. 519:767-772
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Mannoproteins (MPs) are a major component of yeast cell walls and consist of high levels of mannose in covalent complexes with proteins. MPs complexly enhance the immune system. We previously isolated a mutant yeast, K48L3, with a higher yield of MP from its cell wall than wild-type Saccharomyces cerevisiae, YPH499. We determined that K48L3 induces the release of nitric oxide in macrophage cells. The present study reports nitric-oxide-mediated angiogenesis by MP from K48L3 and the induction of the Akt/eNOS signal pathway. Western blotting and RT-PCR were used to demonstrate that MP treatment resulted in the upregulation of p-Akt, p-eNOS, and angiogenesis-mediated gene expression. Moreover, the angiogenesis activity of the MPs was demonstrated using three angiogenesis assays, namely, a cell migration assay, a tube-forming assay, and an ex vivo aorta ring assay. Thus, this study demonstrates for the first time that MPs from S. cerevisiae K48L3 induce angiogenesis in HUVECs via the Akt-eNOS-dependent signaling pathway.
- Subjects :
- 0301 basic medicine
Saccharomyces cerevisiae Proteins
Nitric Oxide Synthase Type III
Angiogenesis
Saccharomyces cerevisiae
Biophysics
Neovascularization, Physiologic
Nitric Oxide
Biochemistry
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Enos
Human Umbilical Vein Endothelial Cells
Humans
Molecular Biology
Protein kinase B
Cells, Cultured
Membrane Glycoproteins
biology
Chemistry
Macrophages
Cell Biology
biology.organism_classification
Cell biology
Endothelial stem cell
Blot
030104 developmental biology
Gene Expression Regulation
030220 oncology & carcinogenesis
Mutation
Signal transduction
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 519
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....ae09c757bb99369ee244882baddf07a1