Back to Search
Start Over
Field evaluation of urine antigen detection for diagnosis of Taenia solium cysticercosis
- Source :
- Transactions of the Royal Society of Tropical Medicine and Hygiene. 105(10)
- Publication Year :
- 2010
-
Abstract
- (Neuro)cysticercosis is an important zoonotic disease caused by infection with Taenia solium metacestode larvae. Existing immunodiagnostic techniques detect antibodies and circulating antigens (Ag) in serum and cerebrospinal fluid (CSF). Blood/CSF collection is an invasive procedure associated with blood-borne infections and is often not well accepted by communities. Detection of circulating Ag in urine has been suggested as an alternative, however this has been evaluated in clinical settings only. The aim of the present study was to evaluate the performance of a urine Ag-ELISA under field conditions. Paired serum and urine samples were obtained from participants in endemic areas of Ecuador (n=748) and Zambia (n=690) and were subjected to a monoclonal antibody-based Ag-ELISA. Calculation of positive and negative agreement indices (AI) showed better agreement in the negative direction both for Ecuadorian and Zambian samples (AI of 93.1 and 86.8, respectively). Using a Bayesian approach to determine the test characteristics, similar sensitivities were obtained for serum and urine Ag detection, whereas a decreased specificity was determined for the urine Ag-ELISA with a lower specificity (78.6%) for Zambian samples than for Ecuadorian samples (88.4%). This study indicates a higher specificity for the serum test under field conditions and promotes further research to improve the urine test.
- Subjects :
- Antigen detection
Male
Performance
Neurocysticercosis
America, Latin
Zambia
Enzyme-Linked Immunosorbent Assay
Urine
Sensitivity and Specificity
Africa, Southern
Sensitivity
Antigen
Taenia solium
parasitic diseases
Diagnosis
medicine
Taeniasis
Animals
Humans
Evaluation
biology
business.industry
Cysticercosis
Rapid diagnostic tests
Public Health, Environmental and Occupational Health
Helminthic diseases
Bayes Theorem
General Medicine
Ag-ELISA
medicine.disease
Metacestode
medicine.drug_formulation_ingredient
Infectious Diseases
Cerebrospinal fluid
Antigens, Helminth
Immunology
biology.protein
Specificity
Parasitology
Monoclonal antibodies
Female
Ecuador
Antibody
business
Subjects
Details
- ISSN :
- 18783503
- Volume :
- 105
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Transactions of the Royal Society of Tropical Medicine and Hygiene
- Accession number :
- edsair.doi.dedup.....ae08f560ff605895295f65259f79eb29