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8-C-(E-phenylethenyl)quercetin from onion/beef soup induces autophagic cell death in colon cancer cells through ERK activation
- Source :
- Molecular nutritionfood research. 61(2)
- Publication Year :
- 2016
-
Abstract
- cope Quercetin, a flavonoid, widely distributed in edible fruits and vegetables, was reported to effectively inhibit 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) formation in a food model (roast beef patties) with itself being converted into a novel compound 8-C-(E-phenylethenyl)quercetin (8-CEPQ). Here we investigated whether 8-CEPQ could be formed in a real food system, and tested its anticancer activity in human colon cancer cell lines. Methods and results LC-MS was applied for the determination of 8-CEPQ formation in onion/beef soup. Anticancer activity of 8-CEPQ was evaluated by using cell viability assay and flow cytometry. Results showed that 8-CEPQ suppressed proliferation and caused G2 phase arrest in colon cancer cells. Based on immunofluorescent staining assay, western blot assay, and RNA knockdown data, we found that 8-CEPQ did not cause apoptotic cell death. Instead, it induced autophagic cell death. Moreover, treatment with 8-CEPQ induced phosphorylation of extracellular signal-regulated kinase (ERK). Inhibition of ERK phosphorylation by the mitogen-activated protein kinase kinase (MEK)/ERK inhibitor U0126 attenuated 8-CEPQ-induced autophagy and reversed 8-CEPQ-mediated cell growth inhibition. Conclusion Our results demonstrate that 8-CEPQ, a novel quercetin derivative, could be formed in onion/beef soup. 8-CEPQ inhibited colon cancer cell growth by inducing autophagic cell death through ERK activation.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
Programmed cell death
Antineoplastic Agents
03 medical and health sciences
chemistry.chemical_compound
Cell Line, Tumor
Onions
Extracellular
Autophagy
Humans
Viability assay
Cooking
Extracellular Signal-Regulated MAP Kinases
Cell Proliferation
Cell Death
Cell growth
Kinase
Cell Cycle Checkpoints
Molecular biology
Enzyme Activation
Red Meat
030104 developmental biology
Biochemistry
chemistry
Cell culture
Colonic Neoplasms
Quercetin
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 16134133
- Volume :
- 61
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular nutritionfood research
- Accession number :
- edsair.doi.dedup.....adff097f28058e3bd145feb07b5fe6cb