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Phospholipase C-Independent Group I Metabotropic Glutamate Receptor-Mediated Inward Current in Mouse Purkinje Cells
- Source :
- Biochemical and Biophysical Research Communications. 251:753-758
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- The mGluR agonist 1S,3R-ACPD induces a potent excitatory inward current in various central neurons, but the underlying mechanism is not fully understood. Thus, we explored the signal transduction mechanism underlying the 1S,3R-ACPD-induced inward current in mouse cerebellar Purkinje cells. Iontophoretic application of 1S,3R-ACPD produced a group I mGluR antagonist-sensitive inward current. This current closely resembled a slow synaptic inward current evoked by repetitive stimulation of parallel fibers. Although phosphoinositide hydrolysis is shown to be coupled with group I mGluRs, we found that intracellular injections of various PLC inhibitors and an IP3 receptor antagonist heparin only partially inhibited the 1S, 3R-ACPD-induced current. Moreover, intracellular injection of the Ca2+ chelator BAPTA or a selective Na+/Ca2+ exchange inhibitor KB-R7943 affected slightly the inward current. In contrast, infusion of GDPbetaS and GTPgammaS markedly suppressed the 1S,3R-ACPD-induced current. These results suggest that activation of mGluR1 in mouse cerebellar Purkinje cells by 1S,3R-ACPD application or by repetitive stimulation of parallel fibres induces an inward current with a minor contribution from intracellular Ca2+ or Na+/Ca2+ exchange.
- Subjects :
- Patch-Clamp Techniques
Biophysics
GTPgammaS
Receptors, Cytoplasmic and Nuclear
Biology
Receptors, Metabotropic Glutamate
Biochemistry
Sodium-Calcium Exchanger
Mice
Purkinje Cells
chemistry.chemical_compound
BAPTA
Image Processing, Computer-Assisted
Animals
Inositol 1,4,5-Trisphosphate Receptors
Cycloleucine
Patch clamp
Egtazic Acid
Evoked Potentials
Molecular Biology
Phospholipase C
Heparin
Electric Conductivity
Thiourea
Cell Biology
Inositol trisphosphate receptor
chemistry
Metabotropic glutamate receptor
Type C Phospholipases
Synapses
Excitatory postsynaptic potential
Metabotropic glutamate receptor 1
Calcium
Calcium Channels
Signal Transduction
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 251
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....adfea7294cae812e0cea36941d9cb2ae