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Long non-coding RNAs associate with jasmonate-mediated plant defence against herbivores
- Source :
- Plant, cellenvironmentREFERENCES. 44(3)
- Publication Year :
- 2020
-
Abstract
- Long non-coding RNA (lncRNA) are important regulators of many biological processes in plants, including defence against pathogens; whether lncRNAs mediate defence against herbivore attack is yet to be explored. With wild tobacco, Nicotiana attenuata, and its well-characterized interactions with herbivores, we identified a total of 1,290 significantly up- or down-regulated lncRNAs in response to a precise herbivore elicitation treatment. Of these, long intergenic non-coding RNAs (lincRNAs) were the most abundant. Based on their expression patterns, these up-regulated lincRNAs were classified as early (1 hr) or late (3 hr) responders. The early responding lincRNAs had accumulation patterns that tracked the herbivore-elicited burst of bioactive jasmonates (JAs) and the expression of regulator genes in JA signalling which regulate plant defences against herbivores. Silencing two of these early responders in N. attenuata (JAL1 and JAL3) significantly attenuated the accumulation of JAs, JA-mediated defensives and the plant's resistance to M. sexta attack, suggesting roles in regulating JA-mediated plant defence. By lincRNA sequencing of JA-deficient lines, many late responder lincRNAs were found to be transcriptionally regulated by JA signalling. This study uncovers a new role of lncRNAs in JA-mediated herbivore resistance.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
Regulator
Plant Science
Cyclopentanes
Biology
01 natural sciences
03 medical and health sciences
Plant Growth Regulators
Gene Expression Regulation, Plant
Nicotiana attenuata
Manduca
Tobacco
Gene silencing
Animals
Jasmonate
Oxylipins
Gene
Genetics
Herbivore
Reverse Transcriptase Polymerase Chain Reaction
RNA
biology.organism_classification
030104 developmental biology
Manduca sexta
RNA, Plant
Plant Defense Against Herbivory
RNA, Long Noncoding
010606 plant biology & botany
Signal Transduction
Subjects
Details
- ISSN :
- 13653040
- Volume :
- 44
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Plant, cellenvironmentREFERENCES
- Accession number :
- edsair.doi.dedup.....adfc3b68d6aaa61ce31e467395133fba