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Development and validation of cross-transferable and polymorphic DNA markers for detecting alien genome introgression in Oryza sativa from Oryza brachyantha
- Source :
- Molecular Genetics and Genomics. 291:1783-1794
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- African wild rice Oryza brachyantha (FF), a distant relative of cultivated rice Oryza sativa (AA), carries genes for pests and disease resistance. Molecular marker assisted alien gene introgression from this wild species to its domesticated counterpart is largely impeded due to the scarce availability of cross-transferable and polymorphic molecular markers that can clearly distinguish these two species. Availability of the whole genome sequence (WGS) of both the species provides a unique opportunity to develop markers, which are cross-transferable. We observed poor cross-transferability (~0.75 %) of O. sativa specific sequence tagged microsatellite (STMS) markers to O. brachyantha. By utilizing the genome sequence information, we developed a set of 45 low cost PCR based co-dominant polymorphic markers (STS and CAPS). These markers were found cross-transferrable (84.78 %) between the two species and could distinguish them from each other and thus allowed tracing alien genome introgression. Finally, we validated a Monosomic Alien Addition Line (MAAL) carrying chromosome 1 of O. brachyantha in O. sativa background using these markers, as a proof of concept. Hence, in this study, we have identified a set molecular marker (comprising of STMS, STS and CAPS) that are capable of detecting alien genome introgression from O. brachyantha to O. sativa.
- Subjects :
- Genetic Markers
0106 biological sciences
0301 basic medicine
DNA, Plant
Introgression
Biology
01 natural sciences
Genome
03 medical and health sciences
chemistry.chemical_compound
Molecular marker
Genetics
Molecular Biology
Crosses, Genetic
DNA Primers
Disease Resistance
Oryza brachyantha
Expressed Sequence Tags
Whole genome sequencing
Polymorphism, Genetic
Oryza sativa
Chromosome Mapping
Reproducibility of Results
food and beverages
Oryza
General Medicine
030104 developmental biology
chemistry
Genetic marker
Microsatellite
Genome, Plant
Microsatellite Repeats
010606 plant biology & botany
Subjects
Details
- ISSN :
- 16174623 and 16174615
- Volume :
- 291
- Database :
- OpenAIRE
- Journal :
- Molecular Genetics and Genomics
- Accession number :
- edsair.doi.dedup.....adfa4a1ba23f69dce03e82503ceea4bc