Polar Arctic Circle biomass enhances performance and stability of aerobic granular sludge systems operated under different temperatures

Three bioreactors were inoculated with Polar Arctic Circle-activated sludge, started-up and operated for 150 days at 8, 15 and 26 °C. Removal performances and granular conformation were similar at steady-state, but higher stability from start-up was found when operating at 8 °C. Important changes in the eukaryotic and prokaryotic populations caused by operational temperature were observed, being fungi dominant at 8 °C and 15 °C, while that ciliated organisms were found at 26 °C. The qPCR results showed higher copies of bacteria, and nitrifiers and denitrifying bacteria at cold temperature. The emission of nitrous oxide was linked directly with temperature and the involved microorganisms. This study represents a proof of concept in performance, greenhouse gas emission, granular formation and the role of the Polar Arctic Circle microbial population in AGS technology under different temperatures with the aim to understand the effect of seasonal o daily changes for implementation of AGS at full-scale.