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Analysis of urinary PSA glycosylation is not indicative of high-risk prostate cancer
- Source :
- Clinica Chimica Acta. 470:97-102
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The levels of core fucosylation and α2,3-linked sialic acid in serum Prostate Specific Antigen (PSA), using the lectins Pholiota squarrosa lectin (PhoSL) and Sambucus nigra agglutinin (SNA), can discriminate between Benign Prostatic Hyperplasia (BPH) and indolent prostate cancer (PCa) from aggressive PCa. In the present work we evaluated whether these glycosylation determinants could also be altered in urinary PSA obtained after digital rectal examination (DRE) and could also be useful for diagnosis determinations. For this purpose, α2,6-sialic acid and α1,6-fucose levels of urinary PSA from 53 patients, 18 biopsy-negative and 35 PCa patients of different aggressiveness degree, were analyzed by sandwich ELLA (Enzyme Linked Lectin Assay) using PhoSL and SNA. Changes in the levels of specific glycosylation determinants, that in serum PSA samples were indicative of PCa aggressiveness, were not found in PSA from DRE urine samples. Although urine is a simpler matrix for analyzing PSA glycosylation compared to serum, an immunopurification step was necessary to specifically detect the glycans on the PSA molecule. Those specific glycosylation determinants on urinary PSA were however not useful to improve PCa diagnosis. This could be probably due to the low proportion of PSA from the tumor in urine samples, which precludes the identification of aberrantly glycosylated PSA.
- Subjects :
- Male
Risk
0301 basic medicine
Glycosylation
Urinary system
Clinical Biochemistry
Urine
urologic and male genital diseases
Risk Assessment
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Prostate cancer
0302 clinical medicine
Agglutinin
medicine
Humans
Fucosylation
Aged
Aged, 80 and over
medicine.diagnostic_test
Biochemistry (medical)
Prostatic Neoplasms
General Medicine
Rectal examination
Middle Aged
Prostate-Specific Antigen
medicine.disease
Sialic acid
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Immunology
Cancer research
Subjects
Details
- ISSN :
- 00098981
- Volume :
- 470
- Database :
- OpenAIRE
- Journal :
- Clinica Chimica Acta
- Accession number :
- edsair.doi.dedup.....adf18251895c176f95f0cd33729c41da