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Crystallization and Preliminary X-ray Studies of l-Aspartase from Escherichia coli

Authors :
Richard D. Kidd
Wuxian Shi
Ronald E. Viola
Gregory K. Farber
John F. Schindler
Francesco Giorgianni
Source :
Journal of Molecular Biology. 234:1248-1249
Publication Year :
1993
Publisher :
Elsevier BV, 1993.

Abstract

Single crystals of L-aspartate ammonia-lyase (L-aspartase) from Escherichia coli have been obtained by microdialysis at room temperature using polyethylene glycol 3350 and sodium acetate as co-precipitants. The crystals exhibit the symmetry of space group P2(1)2(1)2 with a = 156.5 A, b = 147.6 A, c = 102.5 A and diffract at least to 2.8 A.

Details

ISSN :
00222836
Volume :
234
Database :
OpenAIRE
Journal :
Journal of Molecular Biology
Accession number :
edsair.doi.dedup.....ade15b0fecf7488e624cd1b020f09a64
Full Text :
https://doi.org/10.1006/jmbi.1993.1674