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Resonant Acoustic Rheometry to Measure Coagulation Kinetics in Hemophilia A and Healthy Plasma: A Novel Viscoelastic Method

Authors :
Weiping Li
Eric C. Hobson
Connor M. Bunch
Joseph B. Miller
Jimmy Nehme
Hau C. Kwaan
Mark M. Walsh
Michael T. McCurdy
John G. Aversa
Anthony V. Thomas
Nuha Zackariya
Samuel J. Thomas
Stephanie A. Smith
Bernard C. Cook
Bryan Boyd
Jan P. Stegemann
Cheri X. Deng
Source :
Seminars in thrombosis and hemostasis.
Publication Year :
2022

Abstract

Compared with conventional coagulation tests and factor-specific assays, viscoelastic hemostatic assays (VHAs) can provide a more thorough evaluation of clot formation and lysis but have several limitations including clot deformation. In this proof-of-concept study, we test a noncontact technique, termed resonant acoustic rheometry (RAR), for measuring the kinetics of human plasma coagulation. Specifically, RAR utilizes a dual-mode ultrasound technique to induce and detect surface oscillation of blood samples without direct physical contact and measures the resonant frequency of the surface oscillation over time, which is reflective of the viscoelasticity of the sample. Analysis of RAR results of normal plasma allowed defining a set of parameters for quantifying coagulation. RAR detected a flat-line tracing of resonant frequency in hemophilia A plasma that was corrected with the addition of tissue factor. Our RAR results captured the kinetics of plasma coagulation and the newly defined RAR parameters correlated with increasing tissue factor concentration in both healthy and hemophilia A plasma. These findings demonstrate the feasibility of RAR as a novel approach for VHA, providing the foundation for future studies to compare RAR parameters to conventional coagulation tests, factor-specific assays, and VHA parameters.

Details

ISSN :
10989064
Database :
OpenAIRE
Journal :
Seminars in thrombosis and hemostasis
Accession number :
edsair.doi.dedup.....adce4029059cc39cf5af8d784dfa0505