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The Combination of N-Acetyl Cysteine, Alpha-Lipoic Acid, and Bromelain Shows High Anti-Inflammatory Properties in NovelIn VivoandIn VitroModels of Endometriosis
- Source :
- Mediators of Inflammation, Vol 2015 (2015), Mediators of Inflammation
- Publication Year :
- 2015
- Publisher :
- Hindawi Limited, 2015.
-
Abstract
- To evaluate the efficacy of an association of N-acetyl cystein, alpha-lipoic acid, and bromelain (NAC/LA/Br) in the treatment of endometriosis we set up a newin vivomurine model. We explored the anti-inflammatory and proapoptotic effect of this combination on human endometriotic endothelial cells (EECs) and on endothelial cells isolated from normal uterus (UtMECs). We implanted fragments of human endometriotic cysts intraperitoneally into SCID mice to evaluate the efficacy of NAC/LA/Br treatment. UtMECs and EECs, untreated or treated with NAC/LA/Br, were activated with the proinflammatory stimulus TNF-αand their response in terms of VCAM1 expression was evaluated. The proapoptotic effect of higher doses of NAC/LA/Br on UtMECs and EECs was measured with a fluorogenic substrate for activated caspases 3 and 7. The preincubation of EECs with NAC/LA/Br prior to cell stimulation with TNF-αprevents the upregulation of the expression of the inflammatory “marker” VCAM1. Furthermore NAC/LA/Br were able to induce EEC, but not UtMEC, apoptosis. Finally, the novel mouse model allowed us to demonstrate that mice treated with NAC/LA/Br presented a lower number of cysts, smaller in size, compared to untreated mice. Our findings suggest that these dietary supplements may have potential therapeutic uses in the treatment of chronic inflammatory diseases like endometriosis.
- Subjects :
- Pathology
Anti-Inflammatory Agents
Apoptosis
Mice, SCID
Pharmacology
Acetylcysteine
Mice
and bromelain
Endometriosi
Cells, Cultured
Caspase
Microscopy
Cultured
Thioctic Acid
biology
Bromelains
endothelial cells
N-acetyl cystein
endothelial cell
Female
Tumor necrosis factor alpha
Research Article
lcsh:RB1-214
medicine.drug
medicine.medical_specialty
Article Subject
medicine.drug_class
Cells
Immunology
Endometriosis
Vascular Cell Adhesion Molecule-1
SCID
Fluorescence
Anti-inflammatory
NO
Proinflammatory cytokine
In vivo
lcsh:Pathology
medicine
Animals
Humans
Animal
Tumor Necrosis Factor-alpha
Cell Biology
inflammation
alpha-lipoic acid, and bromelain
business.industry
alpha-lipoic acid
Uterus
Disease Models, Animal
Endothelial Cells
Inflammation
Microscopy, Fluorescence
In vitro
Disease Models
biology.protein
business
Subjects
Details
- ISSN :
- 14661861 and 09629351
- Volume :
- 2015
- Database :
- OpenAIRE
- Journal :
- Mediators of Inflammation
- Accession number :
- edsair.doi.dedup.....adb1dd5fb0c0eeaf75ea919afd523e03