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Identification of Structural and Molecular Signatures Mediating Adaptive Changes in the Mouse Kidney in Response to Pregnancy
- Source :
- International Journal of Molecular Sciences; Volume 23; Issue 11; Pages: 6287
- Publication Year :
- 2022
- Publisher :
- MDPI AG, 2022.
-
Abstract
- Pregnancy is characterized by adaptations in the function of several maternal body systems that ensure the development of the fetus whilst maintaining health of the mother. The renal system is responsible for water and electrolyte balance, as well as waste removal. Thus, it is imperative that structural and functional changes occur in the kidney during pregnancy. However, our knowledge of the precise morphological and molecular mechanisms occurring in the kidney during pregnancy is still very limited. Here, we investigated the changes occurring in the mouse kidney during pregnancy by performing an integrated analysis involving histology, gene and protein expression assays, mass spectrometry profiling and bioinformatics. Data from non-pregnant and pregnant mice were used to identify critical signalling pathways mediating changes in the maternal kidneys. We observed an expansion of renal medulla due to proliferation and infiltration of interstitial cellular constituents, as well as alterations in the activity of key cellular signalling pathways (e.g., AKT, AMPK and MAPKs) and genes involved in cell growth/metabolism (e.g., Cdc6, Foxm1 and Rb1) in the kidneys during pregnancy. We also generated plasma and urine proteomic profiles, identifying unique proteins in pregnancy. These proteins could be used to monitor and study potential mechanisms of renal adaptations during pregnancy and disease.
- Subjects :
- Proteomics
kidney
pregnancy
physiology
signalling
renal medulla
mouse urine
mass spectrometry
keratins
proliferation
interstitial cells
Catalysis
Inorganic Chemistry
Mice
Fetus
Pregnancy
Animals
Physical and Theoretical Chemistry
Molecular Biology
Spectroscopy
Kidney Medulla
Organic Chemistry
Proteins
General Medicine
Water-Electrolyte Balance
Computer Science Applications
Female
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 23; Issue 11; Pages: 6287
- Accession number :
- edsair.doi.dedup.....adb045f9be50f8eb55c7438f6fe6ce2b