Back to Search
Start Over
Distinct Functional Interactions between Actin Isoforms and Nonsarcomeric Myosins
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 7, p e70636 (2013)
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- Despite their near sequence identity, actin isoforms cannot completely replace each other in vivo and show marked differences in their tissue-specific and subcellular localization. Little is known about isoform-specific differences in their interactions with myosin motors and other actin-binding proteins. Mammalian cytoplasmic β- and γ-actin interact with nonsarcomeric conventional myosins such as the members of the nonmuscle myosin-2 family and myosin-7A. These interactions support a wide range of cellular processes including cytokinesis, maintenance of cell polarity, cell adhesion, migration, and mechano-electrical transduction. To elucidate differences in the ability of isoactins to bind and stimulate the enzymatic activity of individual myosin isoforms, we characterized the interactions of human skeletal muscle α-actin, cytoplasmic β-actin, and cytoplasmic γ-actin with human myosin-7A and nonmuscle myosins-2A, -2B and -2C1. In the case of nonmuscle myosins-2A and -2B, the interaction with either cytoplasmic actin isoform results in 4-fold greater stimulation of myosin ATPase activity than was observed in the presence of α-skeletal muscle actin. Nonmuscle myosin-2C1 is most potently activated by β-actin and myosin-7A by γ-actin. Our results indicate that β- and γ-actin isoforms contribute to the modulation of nonmuscle myosin-2 and myosin-7A activity and thereby to the spatial and temporal regulation of cytoskeletal dynamics. FRET-based analyses show efficient copolymerization abilities for the actin isoforms in vitro. Experiments with hybrid actin filaments show that the extent of actomyosin coupling efficiency can be regulated by the isoform composition of actin filaments.
- Subjects :
- Macromolecular Assemblies
Cytoplasm
Science
Biophysics
Arp2/3 complex
Actin Filaments
macromolecular substances
Biology
Myosins
Biochemistry
Motor protein
Contractile Proteins
Adenosine Triphosphate
Myosin
Humans
Protein Isoforms
Actin-binding protein
Protein Interaction Maps
Cytoskeleton
Protein Interactions
Muscle, Skeletal
Actin
Multidisciplinary
Physics
Actin remodeling
Proteins
Actins
Cell biology
Cytoskeletal Proteins
Cell Motility
biology.protein
Medicine
Structural Proteins
Cytokinesis
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....ad6f967b654e3595a072c644fd578fdf