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Messenger activity of RNA transcribed in vitro by DNA-RNA polymerase associated to vaccinia virus cores
- Source :
- Journal of Virology. 15:1467-1474
- Publication Year :
- 1975
- Publisher :
- American Society for Microbiology, 1975.
-
Abstract
- The coding properties of RNA transcribed in vitro by purified vaccinia cores have been investigated using Krebs ascites tumor cells, L cells, and reticulocyte lysates. Six to 10 proteins synthesized in vitro are separated on polyacrylamide gels by electrophoresis in the presence of sodium dodecyl sulfate. Their molecular weights vary from 10,000 to 44,000. The electrophoretic behavior of these proteins is similar to that of early proteins isolated from infected L cells. The tryptic peptide analysis of one of these proteins indicates similarity in amino acid sequences. These results show fidelity of both in vitro transcription and molecular weight above 44,000 are synthesized in vitro does not seem due to a competition between 12S mRNA synthesized in excess and RNA of a higher sedimentation coefficient present in a lower amount.
- Subjects :
- Reticulocytes
Transcription, Genetic
Immunology
Vaccinia virus
Sulfur Radioisotopes
Microbiology
Cell Line
Cell-free system
Carcinoma, Krebs 2
Mice
Viral Proteins
L Cells
Methionine
Reticulocyte
Virology
medicine
Protein biosynthesis
Animals
Humans
Trypsin
RNA, Messenger
Polymerase
chemistry.chemical_classification
Messenger RNA
Cell-Free System
biology
Carcinoma
RNA
DNA-Directed RNA Polymerases
Molecular biology
In vitro
Amino acid
medicine.anatomical_structure
chemistry
Protein Biosynthesis
Insect Science
biology.protein
RNA, Viral
Electrophoresis, Polyacrylamide Gel
Mouth Neoplasms
Peptides
Research Article
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....ad6dfdc22c22705dd9a7c7781cfa0329