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Sequencing of 15,622 gene-bearing BACs reveals new features of the barley genome
- Publication Year :
- 2015
- Publisher :
- Cold Spring Harbor Laboratory, 2015.
-
Abstract
- Barley (Hordeum vulgareL.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, since only 6,278 BACs in the physical map were sequenced, detailed fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15,622 BACs representing the minimal tiling path of 72,052 physical mapped gene-bearing BACs. This generated about 1.7 Gb of genomic sequence containing 17,386 annotated barley genes. Exploration of the sequenced BACs revealed that although distal ends of chromosomes contain most of the gene-enriched BACs and are characterized by high rates of recombination, there are also gene-dense regions with suppressed recombination. Knowledge of these deviant regions is relevant to trait introgression, genome-wide association studies, genomic selection model development and map-based cloning strategies. Sequences and their gene and SNP annotations can be accessed and exported via http://harvest-web.org/hweb/utilmenu.wc or through the software HarvEST:Barley (download from harvest.ucr.edu). In the latter, we have implemented a synteny viewer between barley andAegilops tauschiito aid in comparative genome analysis.
- Subjects :
- 0106 biological sciences
2. Zero hunger
Genetics
0303 health sciences
biology
Introgression
food and beverages
Genomics
biology.organism_classification
01 natural sciences
Genome
03 medical and health sciences
Complete sequence
Aegilops tauschii
Hordeum vulgare
Gene
030304 developmental biology
010606 plant biology & botany
Synteny
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ad6dae2b104eb0bfa89b3834e4b06c68
- Full Text :
- https://doi.org/10.1101/018978