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Targeted Photoswitchable Probe for Nanoscopy of Biological Structures
- Source :
- CHEMBIOCHEM, ChemBioChem
- Publication Year :
- 2010
-
Abstract
- SNAP‐tag: We introduce a photoswitchable O6‐benzylguanine derivative and demonstrate its use for super‐resolution microscopy of SNAP‐tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP‐tagged microtubule structures with ∼25 nm resolution. The described probe in combination with the versatile SNAP‐tag labeling opens new possibilities for imaging biological structures at the nanoscale.
- Subjects :
- Guanine
Fluorophore
Nanostructure
Living Cells
Nanotechnology
02 engineering and technology
Fluorophores
Biochemistry
microtubules
03 medical and health sciences
chemistry.chemical_compound
Tubulin
fluorescent probes
Cell Line, Tumor
Storm
Microscopy
Image Processing, Computer-Assisted
Humans
Molecular Biology
Nanoscopic scale
Optical Reconstruction Microscopy
Fluorescent Dyes
030304 developmental biology
SNAP-tag
0303 health sciences
Adenine
Organic Chemistry
Resolution (electron density)
Fusion Proteins
Carbocyanines
021001 nanoscience & nanotechnology
Fluorescence
Optical reconstruction
Nanostructures
3. Good health
Microscopy, Fluorescence
chemistry
Tag
protein structures
Biophysics
Fluorescent-Probes
Molecular Medicine
Resolution
Limit
0210 nano-technology
Subjects
Details
- Volume :
- 11
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- CHEMBIOCHEM
- Accession number :
- edsair.doi.dedup.....ad33f80ea5cff9f7f323bec58fdc6f47