Back to Search
Start Over
microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture
- Source :
- Virology. 448
- Publication Year :
- 2013
-
Abstract
- Infection of Hepatitis B virus (HBV) in hepatocytes has been known to be controlled by multiple cellular factors, while the relationship of the infection and liver microRNAs remains obscure. In this study, a miRNA database, containing 168 unique mature miRNA members from primary hepatocytes of a primate-like animal, northern treeshrew (Tupaia belangeri) that is the only species susceptible for HBV infection other than human and chimpanzee, was established. The relative level of a liver predominant microRNA, miR-122, was markedly increased upon HBV infection of the primary tupaia hepatocyte (PTH). However, introducing neither miR-122 nor its antagonist anti-miR-122 into PTHs, or, HepG2–NTCP that is HepG2 cells with the newly identified receptor sodium taurocholate cotransporting polypeptide (NTCP) did not alter the viral infection on these cells. These data suggest that de novo HBV infection of cultured hepatocytes does not depend on the expression level of intracellular miR-122 of the target cells.
- Subjects :
- Hepatitis B virus
RNA deep sequencing
Tupaia
Pathogenesis
MicroRNA database
De novo infection
medicine.disease_cause
Tupaia belangeri
Virology
microRNA
medicine
MiR-122
Animals
Humans
Hepatocyte
Sodium taurocholate cotransporting polypeptide (NTCP)
Receptor
MicroRNA-122
Cells, Cultured
biology
biology.organism_classification
Hepatitis B
Molecular biology
Disease Models, Animal
MicroRNAs
medicine.anatomical_structure
Liver
Hepatocytes
Subjects
Details
- ISSN :
- 10960341
- Volume :
- 448
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....ad2cf341beb9f66363ffbd458643bd46