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Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging
- Source :
- International Journal of Molecular Sciences, Volume 20, Issue 11, International Journal of Molecular Sciences, Vol 20, Iss 11, p 2825 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Gangliosides are widely expressed in almost all tissues and cells and are also considered to be essential in the development and maintenance of various organs and tissues. However, little is known about their roles in bone metabolism. In this study, we investigated the effects of genetic deletion of ganglioside D3 (GD3) synthase, which is responsible for the generation of all b-series gangliosides, on bone metabolism. Although b-series gangliosides were not expressed in osteoblasts, these gangliosides were expressed in pre-osteoclasts. However, the expression of these gangliosides was decreased after induction of osteoclastogenesis by receptor activator of nuclear factor kappa-B ligand (RANKL). Three-dimensional micro-computed tomography (3D-&mu<br />CT) analysis revealed that femoral cancellous bone mass in GD3 synthase-knockout (GD3S KO) mice was higher than that in wild type (WT) mice at the age of 40 weeks, although there were no differences in that between GD3S KO and WT mice at 15 weeks old. Whereas bone formation parameters (osteoblast numbers/bone surface and osteoblast surface/bone surface) in GD3S KO mice did not differ from WT mice, bone resorption parameters (osteoclast numbers/bone surface and osteoclast surface/bone surface) in GD3S KO mice became significantly lower than those in WT mice at 40 weeks of age. Collectively, this study demonstrates that deletion of GD3 synthase attenuates bone loss that emerges with aging.
- Subjects :
- Aging
medicine.medical_specialty
glycosylation
Osteoclasts
Article
Catalysis
Bone resorption
Bone remodeling
lcsh:Chemistry
Inorganic Chemistry
Mice
Osteogenesis
Osteoclast
Internal medicine
medicine
Animals
Bone Resorption
Physical and Theoretical Chemistry
Receptor
lcsh:QH301-705.5
Molecular Biology
Cells, Cultured
Spectroscopy
Ganglioside
glycosphingolipids
biology
Chemistry
RANK Ligand
Organic Chemistry
Osteoblast
General Medicine
Sialyltransferases
gangliosides
Computer Science Applications
RAW 264.7 Cells
medicine.anatomical_structure
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
RANKL
biology.protein
bone metabolism
Cancellous bone
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....ad2a06dc6d09dbb79c238bd448e8db21