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Diaphragm Repair with a Novel Cross-Linked Collagen Biomaterial in a Growing Rabbit Model
- Source :
- PLoS ONE, PLoS ONE, Vol 10, Iss 7, p e0132021 (2015)
- Publication Year :
- 2015
-
Abstract
- BACKGROUND Neonates with congenital diaphragmatic hernia and large defects often require patch closure. Acellular collagen matrices (ACM) have been suggested as an alternative to synthetic durable patches as they are remodeled by the host or could also be used for tissue engineering purposes. MATERIALS AND METHODS 2.0x1.0 cm diaphragmatic defects were created in 6-weeks old New-Zealand white rabbits. We compared reconstruction with a purpose-designed cross-linked ACM (Matricel) to 4-layer non-cross-linked small intestinal submucosa (SIS) and a 1-layer synthetic Dual Mesh (Gore-Tex). Unoperated animals or animals undergoing primary closure (4/0 polyglecaprone) served as age-matched controls. 60 (n = 25) resp. 90 (n = 17) days later, animals underwent chest x-ray and obduction for gross examination of explants, scoring of adhesion and inflammatory response. Also, uniaxial tensiometry was done, comparing explants to contralateral native diaphragmatic tissue. RESULTS Overall weight nearly doubled from 1,554±242 g at surgery to 2,837±265 g at obduction (+84%). X-rays did show rare elevation of the left diaphragm (SIS = 1, Gore-Tex = 1, unoperated control = 1), but no herniation of abdominal organs. 56% of SIS and 10% of Matricel patches degraded with visceral bulging in four (SIS = 3, Matricel = 1). Adhesion scores were limited: 0.5 (Matricel) to 1 (SIS, Gore-Tex) to the left lung (p = 0.008) and 2.5 (Gore-Tex), 3 (SIS) and 4 (Matricel) to the liver (p
- Subjects :
- Pathology
medicine.medical_specialty
lcsh:Medicine
Diaphragmatic breathing
Adhesion (medicine)
Biocompatible Materials
Gross examination
Tissue engineering
Fibrosis
Medizinische Fakultät
Materials Testing
medicine
Animals
Hernia
ddc:610
lcsh:Science
Hernia, Diaphragmatic
Multidisciplinary
business.industry
lcsh:R
Congenital diaphragmatic hernia
medicine.disease
Diaphragm (structural system)
Surgery
lcsh:Q
Collagen
Rabbits
business
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Vol 10, Iss 7, p e0132021 (2015)
- Accession number :
- edsair.doi.dedup.....acf551a13cef4baa283232412f8dee9b