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Fertilization following mixed insemination with 'cervix-selected' and 'unselected' spermatozoa in the rabbit
- Source :
- Reproduction. 62:337-343
- Publication Year :
- 1981
- Publisher :
- Bioscientifica, 1981.
-
Abstract
- The possible selection of spermatozoa for fertilization by the female genital tract was investigated using genetically homogeneous, numerically adjusted 'cervix-selected' and 'unselected' rabbit spermatozoa. Samples of spermatozoa were marked by 15 min exposure to 1.3 mg TEPA/ml and then washed for 15 min. TEPA-treated and control samples were inseminated alternately into the vagina (= 'cervix-selected') or uterine horns (= 'unselected') of prospective donors. After 6 h spermatozoa were recovered from the uterine horns of the donors. Equal numbers of 'selected' and 'unselected' spermatozoa were inseminated either into the uterine horns (24 does) or oviducts (25 does) of recipients. The fertilization rates were 48 and 72%, respectively. Significantly more eggs were fertilized by untreated than by TEPA-treated spermatozoa. Almost identical fertilization rates, however, were observed between 'cervix-selected' and 'unselected' spermatozoa. It is concluded, therefore, that in the rabbit no selection of (preincubated) spermatozoa for fertilization takes place at the cervical level.
- Subjects :
- Male
Female circumcision
endocrine system
Embryology
Cervix Uteri
Biology
Insemination
Triethylenephosphoramide
Andrology
Endocrinology
Human fertilization
medicine
Animals
Cervix
Insemination, Artificial
reproductive and urinary physiology
urogenital system
Obstetrics and Gynecology
Uterine horns
Cell Biology
Spermatozoa
medicine.anatomical_structure
Reproductive Medicine
Homogeneous
Fertilization
Vagina
Female
Rabbits
Subjects
Details
- ISSN :
- 17417899 and 14701626
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Reproduction
- Accession number :
- edsair.doi.dedup.....acf40a5f8aa2683989d9e213c91f724d