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Pseudogene PTENP1 functions as a competing endogenous RNA (ceRNA) to regulate PTEN expression by sponging miR-499-5p
- Source :
- Biochemistry (Moscow). 81:739-747
- Publication Year :
- 2016
- Publisher :
- Pleiades Publishing Ltd, 2016.
-
Abstract
- Increasing evidence has shown that pseudogenes can widely regulate gene expression. However, little is known about the specific role of PTENP1 and miR-499-5p in insulin resistance. The relative transcription level of PTENP1 was examined in db/db mice and high fat diet (HFD)-fed mice by real-time PCR. To explore the effect of PTENP1 on insulin resistance, adenovirus overexpressing or inhibiting vectors were injected through the tail vein. Bioinformatics predictions and a luciferase reporter assay were used to explore the interaction between PTENP1 and miR-499-5p. The relative transcription level of PTENP1 was largely enhanced in db/db mice and HFD-fed mice. Furthermore, the overexpression of PTENP1 resulted in impaired Akt/GSK activation as well as glycogen synthesis, while PTENP1 inhibition led to the improved activation of Akt/GSK and enhanced glycogen contents. More importantly, PTENP1 could directly bind miR-499-5p, thereby becoming a sink for miR-499-5p. PTENP1 overexpression results in the impairment of the insulin-signaling pathway and may function as a competing endogenous RNA for miR-499-5p, thereby contributing to insulin resistance.
- Subjects :
- Male
0301 basic medicine
Blotting, Western
Mice, Obese
Diet, High-Fat
Real-Time Polymerase Chain Reaction
Biochemistry
Cell Line
Mice
03 medical and health sciences
Genes, Reporter
RNA interference
Transcription (biology)
GSK-3
microRNA
Gene expression
Animals
Insulin
RNA, Small Interfering
Glycogen synthase
3' Untranslated Regions
Protein kinase B
Binding Sites
Base Sequence
biology
Chemistry
Competing endogenous RNA
Glycogen Synthase Kinases
PTEN Phosphohydrolase
General Medicine
Oligonucleotides, Antisense
Molecular biology
Mice, Inbred C57BL
MicroRNAs
030104 developmental biology
biology.protein
RNA Interference
Insulin Resistance
Proto-Oncogene Proteins c-akt
Sequence Alignment
Glycogen
Pseudogenes
Subjects
Details
- ISSN :
- 16083040 and 00062979
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- Biochemistry (Moscow)
- Accession number :
- edsair.doi.dedup.....ace7202ebba7e069750b2653b5fe2a00