Back to Search
Start Over
Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman’s syndrome
- Source :
- Science China Life Sciences. 60:404-416
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Asherman's syndrome (AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells (BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS.
- Subjects :
- 0301 basic medicine
Cell Transplantation
Down-Regulation
Bone Marrow Cells
Asherman's syndrome
Gynatresia
Biology
Real-Time Polymerase Chain Reaction
Endometrium
Peripheral blood mononuclear cell
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Downregulation and upregulation
Environmental Science(all)
medicine
Humans
Pathological
General Environmental Science
Agricultural and Biological Sciences(all)
Tissue Scaffolds
Biochemistry, Genetics and Molecular Biology(all)
Tumor Suppressor Proteins
Regeneration (biology)
medicine.disease
Transplantation
030104 developmental biology
medicine.anatomical_structure
Immunology
Cancer research
Female
Collagen
General Agricultural and Biological Sciences
Collagen scaffold
Transcription Factors
Subjects
Details
- ISSN :
- 18691889 and 16747305
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Science China Life Sciences
- Accession number :
- edsair.doi.dedup.....acd5788fdefdd6e6463bdc79596c50a2