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High-density methylation ofp14ARF andp16INK4A in Epstein-Barr virus-associated gastric carcinoma
- Source :
- International Journal of Cancer. 112:273-278
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- Promoter hypermethylation of various tumor-related genes is extremely frequent in gastric carcinoma (GC) associated with Epstein-Barr virus (EBV). To investigate the significance of the promoter methylation in this type of GC, we examined the methylation densities of the promoter regions of p14ARF and p16INK4A in EBV-associated (n=7) and EBV-negative (n=14) GC. Bisulfite sequencing demonstrated a high frequency of concurrent methylation of p14ARF and p16INK4A promoter regions in EBVaGC. Methylation was observed in all 29 CpG sites of p14ARF and all 16 sites of p16INK4A with equally high densities. In EBV-negative GC, the methylation profiles differed between the 2 genes. Promoter methylation was sporadic and variable in p14ARF, and only the last position of CpG in p14ARF was methylated at high frequency. High-density methylation in p16INK4A was observed in a subset of GC, but the first position of CpG was never methylated in EBV-negative GC. These findings suggest the presence of mechanisms of de novo and maintenance methylation specific to EBVaGC that might be associated with EBV infection.
- Subjects :
- Adult
Male
Epstein-Barr Virus Infections
Cancer Research
Molecular Sequence Data
Bisulfite sequencing
Biology
medicine.disease_cause
Epstein-Barr virus associated gastric carcinoma
p14arf
Stomach Neoplasms
hemic and lymphatic diseases
Tumor Suppressor Protein p14ARF
medicine
Humans
Promoter Regions, Genetic
Aged
Aged, 80 and over
Base Sequence
Genes, p16
Carcinoma
Methylation
DNA Methylation
Middle Aged
Differentially methylated regions
Oncology
CpG site
DNA methylation
Cancer research
Female
Carcinogenesis
Subjects
Details
- ISSN :
- 10970215 and 00207136
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- International Journal of Cancer
- Accession number :
- edsair.doi.dedup.....acb8d675842fd0fe7fec79437274b184