Endogenous cannabinoids in post-mortem brains of Cloninger type 1 and 2 alcoholics

The endogenous cannabinoid (EC) system has been recently implicated in several neuropsychiatric disorders. This study analyzed post-mortem brain regions of Cloninger type 1 (n = 9) and 2 (n = 8) alcoholics and non-alcoholic controls (n = 10) for ECs by quantitative liquid chromatography with triple quadrupole mass spectrometric detection. A significant difference was found in anandamide (AEA) levels in nucleus accumbens (NAcc) between the three groups (p = 0.047). AEA levels were significantly lower when compared to controls in both perigenual anterior cingulate (p = 0.017) and frontal cortices (p = 0.018) of type 1 alcoholics. Similar trends were observed for dihomo-γ-linolenoyl ethanolamide and docosahexaenoyl ethanolamide, but not for 2-arachidonoylglycerol, palmitoyl ethanolamide, or oleoyl ethanolamide. Although preliminary, and from diagnostic groups with a relatively small number of subjects and substantially different mean ages for each group, these results suggest that the EC system may be hyperactive in type 2 alcoholics and hypoactive in type 1 alcoholics.