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Identification and characterization of new miRNAs cloned from normal mouse mammary gland
- Source :
- BMC Genomics, Vol 10, Iss 1, p 149 (2009), BMC Genomics, BMC Genomics april (10, online), Non paginé. (2009), BMC Genomics, BioMed Central, 2009, 10, online (april), Non paginé. ⟨10.1186/1471-2164-10-149⟩
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Background MicroRNAs (miRNAs) are small non-coding RNAs that have been found to play important roles in silencing target genes and that are involved in the regulation of various normal cellular processes. Until now their implication in the mammary gland biology was suggested by few studies mainly focusing on pathological situations allowing the characterization of miRNAs as markers of breast cancer tumour classes. If in the normal mammary gland, the expression of known miRNAs has been studied in human and mice but the full repertoire of miRNAs expressed in this tissue is not yet available. Results To extend the repertoire of mouse mammary gland expressed miRNAs, we have constructed several libraries of small miRNAs allowing the cloning of 455 sequences. After bioinformatics' analysis, 3 known miRNA (present in miRbase) and 33 new miRNAs were identified. Expression of 24 out of the 33 has been confirmed by RT-PCR. Expression of none of them was found to be mammary specific, despite a tissue-restricted distribution of some of them. No correlation could be established between their expression pattern and evolutionary conservation. Six of them appear to be mouse specific. In several cases, multiple potential precursors of miRNA were present in the genome and we have developed a strategy to determine which of them was able to mature the miRNA. Conclusion The cloning approach has allowed improving the repertoire of miRNAs in the mammary gland, an evolutionary recent organ. This tissue is a good candidate to find tissue-specific miRNAs and to detect miRNA specific to mammals. We provide evidence for 24 new miRNA. If none of them is mammary gland specific, a few of them are not ubiquitously expressed. For the first time 6 mouse specific miRNA have been identified.
- Subjects :
- mammary gland
[SDV]Life Sciences [q-bio]
Mammary gland
souris
Proteomics
Mice
Chlorocebus aethiops
Gene expression
Cloning, Molecular
Zebrafish
Genetics
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
030302 biochemistry & molecular biology
Chromosome Mapping
mice
mirna
Haplorhini
Cell biology
medicine.anatomical_structure
COS Cells
micro arn
Female
DNA microarray
Research Article
Biotechnology
lcsh:QH426-470
lcsh:Biotechnology
Molecular Sequence Data
Biology
Transfection
03 medical and health sciences
Dogs
Mammary Glands, Animal
Species Specificity
lcsh:TP248.13-248.65
microRNA
medicine
Animals
Humans
Gene silencing
030304 developmental biology
Cloning
Base Sequence
Gene Expression Profiling
Computational Biology
Blotting, Northern
Chromosomes, Mammalian
Rats
Gene expression profiling
MicroRNAs
lcsh:Genetics
glande mammaire
Chickens
Subjects
Details
- ISSN :
- 14712164
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....acac0523f878258a6aa0b006bc63772d