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Chitinophaga deserti sp. nov., isolated from desert soil
- Source :
- International Journal of Systematic and Evolutionary Microbiology. 69:1783-1788
- Publication Year :
- 2019
- Publisher :
- Microbiology Society, 2019.
-
Abstract
- An aerobic bacterial strain, designated XJ-2T, was isolated from a soil sample collected from Gurbantunggut Sandy Desert in PR China. Cells of strain XJ-2T were Gram-stain-negative, non-motile, rod-shaped and non-spore-forming. The new isolate grew well at 10–37 °C (optimum, 28–30 °C), pH 6.0–11.0 (pH 7.0) and 0–1 % (w/v) NaCl (0 %). The 16S rRNA gene sequence of strain XJ-2T showed the highest similarity to that of Chitinophaga rhizosphaerae T16R-86T (99.0 %), followed by Chitinophaga barathri YLT18T (97.0 %), Chitinophaga humicola Ktm-2T (96.7 %) and Chitinophaga niabensis JS13-10T (96.4 %). The major menaquinone of strain XJ-2T was menaquinone 7 and the predominant fatty acids (>5 %) were iso-C15 : 0, C16 : 1 ω5c and iso-C17 : 0 3-OH. The polar lipids consisted of phosphatidylethanolamine, an unidentified glycolipid, three unidentified aminolipids and five unidentified lipids. The genome size was 6.33 Mb, comprising 5268 predicted genes with a G+C content of 41.5 mol%. The DNA G+C content was 50.5 mol% based on total genome calculations. The average nucleotide identity and the digital DNA–DNA hybridization values between strain XJ-2T and strain T16R-86T were 79.6 and 22.3 %, respectively. DNA–DNA relatedness between strain XJ-2T and strain YLT18T was 17.0 %. Based on the physiological, biochemical and chemotaxonomic characteristics, strain XJ-2T represents a novel species of the genus Chitinophaga , for which the name Chitinophaga deserti sp. nov. is proposed. The type strain is XJ-2T (KCTC 62443T=CCTCC AB 2018019T).
- Subjects :
- DNA, Bacterial
China
Biology
Microbiology
Glycolipid
RNA, Ribosomal, 16S
Chitinophaga niabensis
Chitinophaga barathri
Gene
Genome size
Phylogeny
Soil Microbiology
Ecology, Evolution, Behavior and Systematics
Base Composition
Strain (chemistry)
Bacteroidetes
Phosphatidylethanolamines
Fatty Acids
Nucleic Acid Hybridization
Vitamin K 2
Sequence Analysis, DNA
General Medicine
Genus Chitinophaga
16S ribosomal RNA
Bacterial Typing Techniques
Desert Climate
Subjects
Details
- ISSN :
- 14665034 and 14665026
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- International Journal of Systematic and Evolutionary Microbiology
- Accession number :
- edsair.doi.dedup.....ac9f5148dcd84b096aa25ad9ca6b48a3