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Transfer of skin microbiota between two dissimilar autologous microenvironments: A pilot study
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 12, p e0226857 (2019)
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- Dysbiosis of skin microbiota is associated with several inflammatory skin conditions, including atopic dermatitis, acne, and hidradenitis suppurativa. There is a surge of interest by clinicians and the lay public to explore targeted bacteriotherapy to treat these dermatologic conditions. To date, skin microbiota transplantation studies have focused on moving single, enriched strains of bacteria to target sites rather than a whole community. In this prospective pilot study, we examined the feasibility of transferring unenriched skin microbiota communities between two anatomical sites of the same host. We enrolled four healthy volunteers (median age: 28 [range: 24, 36] years; 2 [50%] female) who underwent collection and transfer of skin microbiota from the forearm to the back unidirectionally. Using culture methods and 16S rRNA V1-V3 deep sequencing, we compared baseline and mixed ("transplant") communities, at T = 0 and T = 24 hours. Our ability to detect movement from one site to the other relied on the inherent diversity of the microenvironment of the antecubital fossa relative to the less diverse back. Comparing bacterial species present in the arm and mixed ("transplant") communities that were absent from the baseline back, we saw evidence of transfer of a partial DNA signature; our methods limit conclusions regarding the viability of transferred organisms. We conclude that unenriched transfer of whole cutaneous microbiota is challenging, but our simple technique, intended to move viable skin organisms from one site to another, is worthy of further investigation.
- Subjects :
- Male
0301 basic medicine
Staphylococcus
Prevotella
Pilot Projects
Pathology and Laboratory Medicine
030207 dermatology & venereal diseases
0302 clinical medicine
RNA, Ribosomal, 16S
Medicine and Health Sciences
Hidradenitis suppurativa
Staphylococcus Aureus
Musculoskeletal System
Acne
Skin
Multidisciplinary
biology
Microbiota
Genomics
Atopic dermatitis
Bacterial Pathogens
3. Good health
Forearm
Arms
Medical Microbiology
Medicine
Female
Anatomy
Pathogens
Bacteriotherapy
Research Article
Adult
Science
Microbial Genomics
Transplantation, Autologous
Microbiology
Young Adult
03 medical and health sciences
Genetics
medicine
Humans
Actinomyces
Microbiome
Staphylococcus Epidermidis
Microbial Pathogens
Back
Bacteria
business.industry
Organisms
Biology and Life Sciences
Corynebacteria
medicine.disease
biology.organism_classification
Transplantation
030104 developmental biology
Body Limbs
Immunology
Dysbiosis
business
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....ac98dc8b6f75b5afe711c76f6c3573e0