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Effect of ammonium formate washing on the elemental composition determination in Nannochloropsis oceanica
- Source :
- Aquaculture 538 (2021), Aquaculture, 538, Aquaculture
- Publication Year :
- 2021
-
Abstract
- This study investigated the effect of salt presence and removal on the integrity and elemental determination of the marine microalgae Nannochloropsis oceanica. Cells were harvested by centrifugation and washed with ammonium formate five times and subjected to mineral analysis after each washing step. Supernatants from each washing step were also analysed. Ca, Cu, Fe, Mg, Mn, Na, P, K, S, Zn were determined by inductively coupled plasma – optical emission spectrometry (ICP-OES). Results showed that washing microalgal biomass twice is sufficient to eliminate equipment oversaturation, allowing for more accurate elemental analysis, by using matrix matching. Moreover, washing the biomass did not rupture the cells, removed cell culture debris and decreased the concentration of Na, K, Ca present in the leftover growth medium. This study provides a reliable protocol for elemental determination in marine microalgae saving time in sample processing and analysis.
- Subjects :
- Bio Process Engineering
Biomass
Salt (chemistry)
Aquatic Science
Biology
Inductively coupled plasma - optical emission spectroscopy (ICP-OES)
Matrix (chemical analysis)
03 medical and health sciences
chemistry.chemical_compound
Ammonium formate
Centrifugation
030304 developmental biology
VLAG
chemistry.chemical_classification
0303 health sciences
Growth medium
Chromatography
Sodium
04 agricultural and veterinary sciences
Minerals in microalgae
Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 [VDP]
chemistry
Elemental analysis
Elemental composition
040102 fisheries
0401 agriculture, forestry, and fisheries
Environmental Technology
Milieutechnologie
Inductively coupled plasma
Subjects
Details
- Language :
- English
- ISSN :
- 00448486
- Volume :
- 538
- Database :
- OpenAIRE
- Journal :
- Aquaculture
- Accession number :
- edsair.doi.dedup.....ac930ab99c881431d69d76742f776b51