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Media composition modulates human embryonic stem cell morphology and may influence preferential lineage differentiation potential
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 3, p e0213678 (2019)
- Publication Year :
- 2018
-
Abstract
- Undifferentiated human embryonic stem cells have a distinct morphology (hESC). Changes in cell morphology during culture can be indicative of differentiation. hESC, maintained in diverse medias, demonstrated alterations in morphological parameters and subsequent alterations in underlying transcript expression and lineage differentiation. Analysis of morphological parameters showed distinct and significant differences between the undefined, less defined and Xeno-free medias while still maintaining pluripotency markers. This suggested that the less defined media may be creating dynamic instability in the cytoskeleton, with the cytoskeleton becoming more stabilised in the Xeno-free media as demonstrated by smaller and rounder cells. Examination of early lineage markers during undirected differentiation using d5 embryoid bodies demonstrated increased mesodermal lineage preference as compared to endodermal or ectoderm in cells originally cultured in Xeno-free media. Undefined media showed preference for mesoderm and ectoderm lineages, while less defined media (BSA present) demonstrated no preference. These data reveal that culture media may produce fundamental changes in cell morphology which are reflected in early lineage differentiation choice.
- Subjects :
- 0301 basic medicine
Embryology
Transcription, Genetic
Cellular differentiation
Human Embryonic Stem Cells
Cell Culture Techniques
Gene Expression
Ectoderm
Embryoid body
Mesoderm
0302 clinical medicine
Animal Cells
Cluster Analysis
Cytoskeleton
Staining
Multidisciplinary
Stem Cells
Endoderm
Cell Staining
Cell Differentiation
Cell biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
embryonic structures
Medicine
Cellular Structures and Organelles
Cellular Types
Research Article
Pluripotent Stem Cells
Pluripotency
Lineage (genetic)
Adhesion Molecules
Science
Cell Potency
Biology
Research and Analysis Methods
Cell Line
03 medical and health sciences
medicine
Genetics
Humans
Cell Lineage
Embryoid Bodies
Focal Adhesions
Lineage markers
Gene Expression Profiling
Biology and Life Sciences
Cell Biology
Molecular Development
Embryonic stem cell
Culture Media
030104 developmental biology
Microscopy, Fluorescence
Specimen Preparation and Treatment
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....ac68cf122bd7f08d3da63a9bfb8b72d0