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Binding of Pseudomonas aeruginosa to neutral glycosphingolipids of rabbit corneal epithelium
- Source :
- Infection and Immunity. 58:114-118
- Publication Year :
- 1990
- Publisher :
- American Society for Microbiology, 1990.
-
Abstract
- 35S-labeled Pseudomonas aeruginosa isolates were shown to bind to neutral glycosphingolipids (NGSLs) of rabbit corneal epithelia in culture by a thin-layer chromatogram overlay procedure. The lipids of the corneal epithelial cells grown in culture were extracted and partitioned into a chloroform-rich lower phase containing NGSLs and an aqueous upper phase containing gangliosides. By using a dot-blot assay, at least six times more radiolabeled P. aeruginosa isolates were shown to bind to the lipids in the lower phase compared with those in the upper phase. Thin-layer chromatography of the lower-phase lipids followed by staining with an orcinol spray revealed at least 10 NGSL components and several fast-migrating, nonglycosylated neutral lipid components (including cholesterol). 35S-labeled P. aeruginosa was shown to bind to NGSL components 1, 2, 5, 6, and 9. P. aeruginosa-reactive NGSL components 6 and 9 migrated with chromatographic mobilities similar to those of the standards ceramide trihexoside (CT) and ceramide monohexoside, respectively. Components 1 and 2 migrated slightly ahead of asialo GM1, and component 5 migrated faster than globoside but slower than CT. Among the various standards tested, P. aeruginosa bound to asialo GM1 and, to a lesser extent, to ceramide dihexoside and CT but not to GM1, GD1A, GM3, or ceramide monohexoside. It remains to be determined whether any of the five P. aeruginosa-reactive NGSL components of corneal epithelium identified in this study plays a role in the development of corneal infection. However, we have previously shown that component 9, one of the five P. aeruginosa-reactive NGSL components identified in this study, is present in significantly greater amounts in migrating epithelia than it is in nonmigrating epithelia (N. Panjwani, G. Michalopoulos, J. Song, G. Yogeeswaran, and J. Baum, Invest. Ophthalmol. Vis. Sci., in press). This may prove to be of biological significance because it is generally believed that traumatized (migrating) epithelia are more susceptible to infection than normal (nonmigrating) epithelia are.
- Subjects :
- Immunology
In Vitro Techniques
medicine.disease_cause
Orcinol
Microbiology
Bacterial Adhesion
Epithelium
Glycosphingolipids
Cornea
chemistry.chemical_compound
medicine
Animals
Corneal epithelium
Lagomorpha
biology
Globoside
Pseudomonas aeruginosa
biology.organism_classification
Staining
Cholesterol
Infectious Diseases
medicine.anatomical_structure
chemistry
Biochemistry
lipids (amino acids, peptides, and proteins)
Parasitology
Chromatography, Thin Layer
Rabbits
Research Article
Pseudomonadaceae
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....ac5730844453ffd60142bbe64d771ba6