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The effect of sonication on simulated osteoarthritis. Part I: Effects of 1 MHz ultrasound on uptake of hyaluronan into the rabbit synovium
- Source :
- Ultrasound in Medicine & Biology. 31:1551-1558
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- High molecular weight (MW) hyaluronan (HA) preparation is considered to be more biologically active than HAs of lower MWs. However, many of the HA preparations currently used to treat osteoarthritis (OA) have lower MWs by the enhanced penetration of HA molecules into the synovial lining cells. In this study, we determined the effectiveness of sonophoresis on the delivery of high MW HA into synovial membrane using an animal model of OA. A total of 1000 kDa (HA1000) and 3000 kDa (HA3000) HA were labeled with fluorescein and injected into the knees of rabbits. Low-intensity continuous ultrasound at 1 MHz, 400 mW/cm2 was applied to the knees for 10 min treatment bid. Synovial fluid analysis revealed increased absorption and fluorescence microscopy showed deeper penetration of both HA1000 and HA3000, more so with the latter. Histologic examination indicated that ultrasound treatment resulted in no apparent damage to the synovial membrane. These results suggest that simultaneous sonication with HA injection might compensate for the short half-life of HA. Consequently, this dual treatment would render HA a far more effective tool in the management of OA.
- Subjects :
- Pathology
medicine.medical_specialty
Acoustics and Ultrasonics
Sonication
Biophysics
Osteoarthritis
chemistry.chemical_compound
Synovial Fluid
medicine
Fluorescence microscope
Animals
Radiology, Nuclear Medicine and imaging
Hyaluronic Acid
Fluorescein
Radiological and Ultrasound Technology
business.industry
Synovial Membrane
Ultrasound
Penetration (firestop)
Osteoarthritis, Knee
medicine.disease
Sonophoresis
Hindlimb
Molecular Weight
medicine.anatomical_structure
chemistry
Models, Animal
Female
Rabbits
Synovial membrane
business
Half-Life
Biomedical engineering
Subjects
Details
- ISSN :
- 03015629
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Ultrasound in Medicine & Biology
- Accession number :
- edsair.doi.dedup.....ac5368a7037621db555d1ab5578a1b5b