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Investigation into the possibility of vertical transmission of avian bornavirus in free-ranging Canada geese (Branta canadensis)
- Source :
- Avian pathology : journal of the W.V.P.A. 43(4)
- Publication Year :
- 2014
-
Abstract
- To investigate the possibility of in ovo infection with avian bornavirus (ABV) in wild Canada geese (Branta canadensis), 53 eggs were opportunistically collected at various stages of embryonic development from 16 free-ranging goose nests at a large urban zoo site where ABV infection is known to be present in this species. ABV RNA was detected in the yolk of one of three unembryonated eggs using real-time reverse transcription polymerase chain reaction. ABV RNA was not identified in the brains from 23 newly hatched goslings or 19 embryos, nor from three early whole embryos. Antibodies against ABV were not detected in the plasma of any of the hatched goslings using an enzyme-linked immunosorbent assay. Possible reasons for the failure to detect ABV RNA in hatchlings or embryos include low sample size, eggs deriving from parents not actively infected with ABV, the testing of only brain tissue, and failure of the virus to replicate in Canada goose embryos. In conclusion, this preliminary investigation demonstrating the presence of ABV RNA in the yolk of a Canada goose egg provides the first evidence for the potential for vertical transmission of ABV in waterfowl.
- Subjects :
- animal structures
food.ingredient
business.operation
Zoology
In ovo
Real-Time Polymerase Chain Reaction
Branta
Virus
Goose
food
Food Animals
biology.animal
Yolk
Geese
Waterfowl
Animals
Hatchling
Ovum
General Immunology and Microbiology
biology
Ecology
Bird Diseases
Reverse Transcriptase Polymerase Chain Reaction
Mononegavirales Infections
biology.organism_classification
Infectious Disease Transmission, Vertical
Canada goose
Bornaviridae
embryonic structures
RNA, Viral
Animal Science and Zoology
business
Subjects
Details
- ISSN :
- 14653338
- Volume :
- 43
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Avian pathology : journal of the W.V.P.A
- Accession number :
- edsair.doi.dedup.....ac39c2aaddb6fcb9e88efd8316e85022