Back to Search
Start Over
Honokiol induces cell apoptosis in human chondrosarcoma cells through mitochondrial dysfunction and endoplasmic reticulum stress
- Source :
- Cancer letters. 291(1)
- Publication Year :
- 2009
-
Abstract
- Chondrosarcoma is a malignant primary bone tumor that responds poorly to both chemotherapy and radiation therapy. In the present study, we investigated the anti-cancer effect of a honokiol, an active component isolated and purified from the Magnolia officinalis in human chondrosarcoma cells. Honokiol-induced cell apoptosis in human chondrosarcoma cell lines (including: JJ012 and SW1353) but not primary chondrocytes. Honokiol also induces upregulation of Bax and Bak, downregulation of Bcl-XL and dysfunction of mitochondria in chondrosarcoma cells. Honokiol triggered endoplasmic reticulum (ER) stress, as indicated by changes in cytosol-calcium levels. We also found that honokiol increased the expression and activities of glucose-regulated protein 78 (GRP78) and calpain. Transfection of cells with GRP78 or calpain siRNA reduced honokiol-mediated cell apoptosis in JJ012 cells. Importantly, animal studies have revealed a dramatic 53% reduction in tumor volume after 21 days of treatment. This study demonstrates that honokiol may be a novel anti-cancer agent targeting chondrosarcoma cells.
- Subjects :
- Honokiol
Male
Cancer Research
Chondrosarcoma
Apoptosis
Bone Neoplasms
Biology
Endoplasmic Reticulum
Lignans
chemistry.chemical_compound
Mice
Downregulation and upregulation
Cell Line, Tumor
medicine
Animals
Humans
Endoplasmic Reticulum Chaperone BiP
Heat-Shock Proteins
Mice, Inbred BALB C
Calpain
Endoplasmic reticulum
Biphenyl Compounds
Transfection
medicine.disease
Antineoplastic Agents, Phytogenic
Xenograft Model Antitumor Assays
Cell biology
Mitochondria
Oncology
chemistry
Cell culture
biology.protein
Calcium
Subjects
Details
- ISSN :
- 18727980
- Volume :
- 291
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cancer letters
- Accession number :
- edsair.doi.dedup.....ac371c9ac0fec6df60d175061d191458