Back to Search
Start Over
Extent of reduction of foot-and-mouth disease virus RNA load in oesophageal–pharyngeal fluid after peak levels may be a critical determinant of virus persistence in infected cattle
- Source :
- Journal of General Virology. 85:415-421
- Publication Year :
- 2004
- Publisher :
- Microbiology Society, 2004.
-
Abstract
- To investigate whether foot-and-mouth disease virus (FMDV) RNA loads in oesophageal–pharyngeal fluid (OP-fluid) in the early course of infection is related to the outcome of virus persistence, viral RNA in OP-fluid samples from cattle experimentally infected with FMDV type O was quantitatively analysed by using a quantitative real-time RT-PCR. Viral RNA was detected within 24 h post-infection (p.i.) in all infected animals. Rapid virus replication led to peak levels of viral RNA load by 30–53 h p.i., and then the load declined at various rates. In some animals (n=12, so-called non-carriers) viral RNA became undetectable between 7 and 18 days p.i. In contrast, in persistently infected animals (n=12, so-called carriers) viral RNA persisted in OP-fluid samples at detectable levels beyond 28 days p.i. Analysis of early viral decay/clearance and virus clearance half-life in OP-fluid samples showed that the extent of reduction of viral RNA in OP-fluid samples immediately following peak levels is a critical determinant of the outcome of FMDV persistence.
- Subjects :
- viruses
Virus
Persistence (computer science)
Esophagus
Virology
medicine
Animals
Therapeutic Irrigation
Aphthovirus
Foot-and-mouth disease
biology
Reverse Transcriptase Polymerase Chain Reaction
RNA
Viral Load
medicine.disease
biology.organism_classification
Body Fluids
Viral replication
Foot-and-Mouth Disease Virus
Foot-and-Mouth Disease
Carrier State
Pharynx
RNA, Viral
Cattle
Foot-and-mouth disease virus
Viral load
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....ac296992198e5cb5c8f56f5e7a4cd51b
- Full Text :
- https://doi.org/10.1099/vir.0.19538-0