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N-Cadherin and M-Cadherin are sequentially expressed in myoblast populations contributing to the first and second waves of myogenesis in the trout (Oncorhynchus mykiss)

Authors :
Véronique Lebret
Cécile Rallière
Pierre-Yves Rescan
Laboratoire de Physiologie et Génomique des Poissons (LPGP)
Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS)
Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)
Source :
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Wiley, 2012, 318B (1), pp.71-77. ⟨10.1002/jez.b.21443⟩
Publication Year :
2012
Publisher :
HAL CCSD, 2012.

Abstract

The objective of this study was to investigate the expression of two promyogenic cell surface adhesion receptors, N- and M-cadherin, in developing trout (Oncorhynchus mykiss) somite, taking account of the recent identification of a dermomyotome-like epithelium in teleosts. In situ hybridization showed that N-cadherin was expressed throughout the paraxial mesoderm and nascent somite. As the somite matured, N-cadherin expression disappeared ventrally from the sclerotome, and then mediolaterally from the differentiating slow and fast muscle cells of the embryonic myotome, to become finally restricted to the undifferentiated myogenic precursors forming the dermomyotome-like epithelium that surrounds the embryonic myotome. By contrast, M-cadherin, which was transcribed in the differentiating embryonic myotome, was never expressed in the dermomyotome-like epithelium. In late-stage trout embryos, M-cadherin transcript was only detected at the periphery of the expanding myotome, where muscle cells stemming from the N-cadherin positive dermomyotome-like epithelium differentiate. Collectively, our results support the view that, in trout embryo, N-cadherin is associated with muscle cell immaturity while M-cadherin is associated with muscle cell maturation and differentiation and this during the two successive phases of myogenesis. J. Exp. Zool. (Mol. Dev. Evol.) 318:71-77, 2012. (C) 2011 Wiley Periodicals, Inc.

Details

Language :
English
ISSN :
15525007 and 15525015
Database :
OpenAIRE
Journal :
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Wiley, 2012, 318B (1), pp.71-77. ⟨10.1002/jez.b.21443⟩
Accession number :
edsair.doi.dedup.....ac1b01eb9d2cb167a2e95c5a499175a0
Full Text :
https://doi.org/10.1002/jez.b.21443⟩