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Interaction of Class I Human Leukocyte Antigen (HLA-I) Molecules with Insulin Receptors and Its Effect on the Insulin-Signaling Cascade
- Source :
- Molecular Biology of the Cell. 8:2463-2474
- Publication Year :
- 1997
- Publisher :
- American Society for Cell Biology (ASCB), 1997.
-
Abstract
- Insulin receptor (IR) and class I major histocompatibility complex molecules associate with one another in cell membranes, but the functional consequences of this association are not defined. We found that IR and human class I molecules (HLA-I) associate in liposome membranes and that the affinity of IR for insulin and its tyrosine kinase activity increase as the HLA:IR ratio increases over the range 1:1 to 20:1. The same relationship between HLA:IR and IR function was found in a series of B-LCL cell lines. The association of HLA-I and IR depends upon the presence of free HLA heavy chains. All of the effects noted were reduced or abrogated if liposomes or cells were incubated with excess HLA-I light chain, β2-microglobulin. Increasing HLA:IR also enhanced phosphorylation of insulin receptor substrate-1 and the activation of phosphoinositide 3-kinase. HLA-I molecules themselves were phosphorylated on tyrosine and associated with phosphoinositide 3-kinase when B-LCL were stimulated with insulin.
- Subjects :
- Insulin Receptor Substrate Proteins
medicine.medical_treatment
Human leukocyte antigen
Fluorescence
Article
Cell Line
Phosphatidylinositol 3-Kinases
Enzyme activator
Adenosine Triphosphate
Insulin receptor substrate
HLA-A2 Antigen
medicine
Humans
Insulin
Glycophorins
Phosphorylation
Phosphotyrosine
Molecular Biology
Fluorescent Dyes
B-Lymphocytes
biology
Cell Biology
Phosphoproteins
Molecular biology
Receptor, Insulin
Enzyme Activation
Kinetics
Insulin receptor
Phenotype
Energy Transfer
Liposomes
biology.protein
Thermodynamics
Signal transduction
beta 2-Microglobulin
Tyrosine kinase
Protein Binding
Signal Transduction
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....ac019917cf80688e22b41282fb5a607c